PXD040426

PXD040426 is an original dataset announced via ProteomeXchange.

Title	Identificaiton of novel acetylation sites in UHRF1 mediated by acetyltransferase MOF
Description	The multi-domain protein UHRF1 (ubiquitin-like, containing PHD and RING finger domains, 1) recruits DNMT1 for DNA methylation maintenance during DNA replication. Here, we show that MOF (Males absent On the First) is an acetyltransferase of UHRF1 to acetylate UHRF1 at Lys670 in the pre-RING linker region whereas HDAC1 is a deacetylase of UHRF1 at the same site. The MOF/HDAC1-mediated acetylation in UHRF1 is cell-cycle regulated and peaks at G1/S phase, in line with the function of UHRF1 in recruiting DNMT1 to maintain DNA methylation. In addition, UHRF1 acetylation significantly enhances its E3 ligase activity and elimination of UHRF1 acetylation at these sites attenuates UHRF1-mediated H3 ubiquitination, which in turn impairs the DNMT1 recruitment and DNA methylation. Taken together, these findings not only identify MOF as a new acetyltransferase for UHRF1 but also reveal a novel mechanism underlying the regulation of DNA methylation maintenance through MOF-mediated UHRF1 acetylation.
HostingRepository	PRIDE
AnnounceDate	2024-03-06
AnnouncementXML	Submission_2024-03-05_20:01:36.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linsheng WANG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 05:18:56	ID requested
⏵ 1	2024-03-05 20:01:36	announced

10.1016/J.CELREP.2024.113908;

submitter keyword: Acetylation

UHRF1

MOF

Zhongjun ZHOU
contact affiliation	School of biomedical sciences, the university of Hong Kong
contact email	zhongjun@hku.hk
lab head
Linsheng WANG
contact affiliation	Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences),
contact email	wanglsh@connect.hku.hk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD040426

PRIDE project URI

• PRIDE
  1. PXD040426
     1. Label: PRIDE project
     2. Name: Identificaiton of novel acetylation sites in UHRF1 mediated by acetyltransferase MOF