PXD040415

PXD040415 is an original dataset announced via ProteomeXchange.

Title	LC-MS experiments of SARS-CoV2 Receptor Binding Domain WT
Description	Antigen specific humoral immunity can be characterized by the analysis of serum antibodies. While serological assays for the measurement of antibody levels and of neutralization potential against SARS-CoV-2 are available, these are not quantitative in the biochemical sense. Yet, understanding the biology of COVID-19 would need an unambiguous, complete, quantitative, comparable measurement of specific serum antibodies. Here we describe a fluorescent, dual-titration immunoassay, which provides the physico-chemical parameters that are both necessary and sufficient to quantitatively characterize the humoral immune response. We used recombinant Receptor Binding Domain of SARS-CoV-2 as antigen on microspot arrays and varied the concentration of both the antigen and serum antibodies from vaccinated persons to obtain a measurement matrix of binding data. Binding curves were fitted using a novel algorithm to obtain thermodynamic variables of binding. We defined the standard state for a system of serum antibodies and antigen and showed how a normalized generalized logistic function is related to thermodynamic activity, standard concentration and activity coefficient. The utility of the method is demonstrated by defining the composition of tested sera with respect to immunoglobulin classes, affinity, concentration, and thermodynamic activity. The proposed fluorescent dual-titration microspot immunoassay can generate truly quantitative serological data that is suitable for immunological, medical and systems biological analysis.
HostingRepository	PRIDE
AnnounceDate	2025-02-21
AnnouncementXML	Submission_2025-02-21_05:33:49.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Nyberg Larsen
SpeciesList	scientific name: recombinant SARS coronavirus; NCBI TaxID: 575560;
ModificationList	carbamoylated residue; monohydroxylated residue; deaminated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 04:29:48	ID requested
⏵ 1	2025-02-21 05:33:49	announced

10.3389/FIMMU.2025.1494624;

submitter keyword: SARS-CoV2, RBD,LC-MSMS

Peter Højrup
contact affiliation	University of Southern Denmark, Faculty of Science, Departments of Biochemistry and Molecular Biology
contact email	php@bmb.sdu.dk
lab head
Daniel Nyberg Larsen
contact affiliation	University of Southern Denmark
contact email	daniel.nyberg@live.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040415
     1. Label: PRIDE project
     2. Name: LC-MS experiments of SARS-CoV2 Receptor Binding Domain WT