PXD040410

PXD040410 is an original dataset announced via ProteomeXchange.

Title	Proteomes of Spirodela polyhriza under different trophic modes
Description	The greater duckweed (Spirodela polyrhiza 7498) exhibits trophic diversity (photoautotrophic, heterotrophic, photoheterotrophic, and mixotrophic growth) depending on the availability of exogenous organic carbon sources and light. Here, we show that the ability to transition between various trophic growth conditions is an advantageous trait, providing great phenotypic plasticity and metabolic flexibility in S. polyrhiza 7498. By comparing S. polyrhiza 7498 growth characteristics, metabolic acclimation, and cellular ultrastructure across these trophic modes, we show that mixotrophy decreases photosynthetic performance and relieves the CO2 limitation of photosynthesis by enhancing the CO2 supply through the active respiration pathway. Proteomic and metabolomic analyses corroborated that S. polyrhiza 7498 increases its intracellular CO2 and decreases reactive oxygen species undermixotrophic and heterotrophic conditions, which substantially suppressed the wasteful photorespiration and oxidative-damage pathways. As a consequence, mixotrophy resulted in a higher biomass yield than the sum of photoautotrophy and heterotrophy.Our work provides a basis for using trophic transitions in S. polyrhiza 7498 for the enhanced accumulation of value-added products.
HostingRepository	iProX
AnnounceDate	2023-02-27
AnnouncementXML	Submission_2023-03-20_00:29:02.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zuoliang Sun
SpeciesList	scientific name: Spirodela polyrhiza; NCBI TaxID: 29656;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-27 00:02:13	ID requested
1	2023-02-27 00:02:31	announced
⏵ 2	2023-03-20 00:29:03	announced	2023-03-20: Update publication information.

Sun Z, Zhao X, Li G, Yang J, Chen Y, Xia M, Hwang I, Hou H, Metabolic flexibility during a trophic transition reveals the phenotypic plasticity of greater duckweed (Spirodela polyrhiza 7498). New Phytol, 238(4):1386-1402(2023) [pubmed]

submitter keyword: Spirodela polyhriza, duckweed, trophic mode, metabolic flexibility, phenotypic plasticity, new crop

Hongwei Hou
contact affiliation	Institute of Hydrobiology, Chinese Academy of Sciences
contact email	houhw@ihb.ac.cn
lab head
Zuoliang Sun
contact affiliation	Institute of Hydrobiology, Chinese Academy of Sciences
contact email	zlsun@ihb.ac.cn
dataset submitter

iProX dataset URI