PXD040407

PXD040407 is an original dataset announced via ProteomeXchange.

Title	H3K79 methyltransferase Dot1p/DOT1L is phosphorylated by proline-directed MAP kinases Hog1p and p38/MAPK11
Description	In vitro phosphorylation assays were conducted to identify phosphorylation sites on Dot1p catalysed by Hog1p. As several other kinases were co-purified with Hog1p, dead kinase control assays (Hog1p-K52R and Hog1p-D144A) were performed to verify the identified Dot1p phospho-sites. This entry contains the data pertaining to the in vitro Hog1p dead kinase phosphorylation assays, which is in supplement to existing PRIDE entry PXD036747.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-28_18:22:11.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Separovich
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-26 22:41:34	ID requested
⏵ 1	2024-01-28 18:22:12	announced

Separovich RJ, Karakatsanis NM, Gao K, Fuh D, Hamey JJ, Wilkins MR, Proline-directed yeast and human MAP kinases phosphorylate the Dot1p/DOT1L histone H3K79 methyltransferase. FEBS J, 291(12):2590-2614(2024) [pubmed]

10.1111/febs.17062;

submitter keyword: histone methyltransferase, yeast,phosphorylation

Dr Marc R Wilkins
contact affiliation	Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia
contact email	m.wilkins@unsw.edu.au
lab head
Ryan Separovich
contact affiliation	University of New South Wales
contact email	r.separovich@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD040407

PRIDE project URI

• PRIDE
  1. PXD040407
     1. Label: PRIDE project
     2. Name: H3K79 methyltransferase Dot1p/DOT1L is phosphorylated by proline-directed MAP kinases Hog1p and p38/MAPK11