PXD040406

PXD040406 is an original dataset announced via ProteomeXchange.

Title	Identification of Protein Biomarkers in Toad Venom from Bufo gargarizans
Description	Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of it has obvious limitation for lack of protein study. Thus, it’s necessary to screen suitable biomarkers and establish appropriate quality evaluation method for the characteristics of toad venom’s proteins, guaranteeing the safety and efficacy in clinical applications. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis, high-performance liquid chromatography and cytotoxicity assay were used to analyze protein components difference between toad venom of different areas. Functional proteins were screened as potential biomarkers by tandem mass tags proteomic and bioinformatic analysis. The results showed that the composition of different toad venom was similar. The protein content of Lianyungang’s toad venom was higher and the alcohol-soluble substance was lower, which suggested that they had no correlation. Simultaneously, the protein component was verified to have strong cytotoxicity. Proteomics analysis showed that 13 antimicrobial proteins, 4 anti-inflammatory and analgesic proteins, and 20 antitumor proteins were screened in the extracellular differentially expressed proteins. A candidate list of functional proteins that could be used as potential biomarkers was presented. Moreover, the antimicrobial activity of Lysozyme C-1 and the anti-inflammatory and analgesic activity of Neuropeptide B were identified as biomarkers for toad venom protein in future. The biomarkers can form the basis for the quality studies of toad venom’s proteins, helping construct and improve the safe, scientific and comprehensive quality evaluation method.
HostingRepository	iProX
AnnounceDate	2023-02-27
AnnouncementXML	Submission_2023-05-06_00:08:11.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meiyun Yang
SpeciesList	scientific name: Bufo gargarizans; NCBI TaxID: 30331;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-26 21:36:20	ID requested
1	2023-02-26 21:36:35	announced
⏵ 2	2023-05-06 00:08:12	announced	2023-05-06: Update publication information.

Yang M, Huan W, Zhang G, Li J, Xia F, Durrani R, Zhao W, Lu J, Peng X, Gao F, . Molecules, 28(8):(2023) [pubmed]

submitter keyword: Bufo gargarizans, ear-side glands, toad venom, extracellular proteins, function, biomarker

Fei Gao
contact affiliation	Zhejiang A&F University
contact email	20140003@zafu.edu.cn
lab head
Meiyun Yang
contact affiliation	Zhejiang A&F University
contact email	2788692769@qq.com
dataset submitter

iProX dataset URI