PXD040405
PXD040405 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RNA MS2-tag based affinity method was used to purify 3' processing factors associated with intronic PAS processing |
| Description | three copies of the MS2 hairpin were fused to the intronic PAS of the nr3c1 gene. To make a negative control RNA substrate, the core hexamer AAUAAA within PAS was mutated to AACAAA. Subsequently, the RNA substrate was incubated with HeLa NE, and the mRNA 3' processing complex was purified using an MS2 tag |
| HostingRepository | iProX |
| AnnounceDate | 2023-02-27 |
| AnnouncementXML | Submission_2023-08-29_21:48:23.151.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chengguo Yao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-26 21:33:41 | ID requested | |
| 1 | 2023-02-26 21:34:19 | announced | |
| ⏵ 2 | 2023-08-29 21:48:23 | announced | 2023-08-30: Update publication information. |
Publication List
| Feng Q, Lin Z, Deng Y, Ran Y, Yu R, Xiang AP, Ye C, Yao C, The U1 antisense morpholino oligonucleotide (AMO) disrupts U1 snRNP structure to promote intronic PCPA modification of pre-mRNAs. J Biol Chem, 299(7):104854(2023) [pubmed] |
Keyword List
| submitter keyword: Intronic PAS processing factors identification |
Contact List
| Peng Xiang | |
|---|---|
| contact affiliation | Sun Yat-sen University |
| contact email | xiangp@mail.sysu.edu.cn |
| lab head | |
| Chengguo Yao | |
| contact affiliation | Sun Yat-sen University |
| contact email | yaochguo@mail.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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