⮝ Full datasets listing

PXD040383

PXD040383 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe role of serine/threonine-specific protein kinases in cyanobacteria - SpkB is involved in acclimation to fluctuating conditions in Synechocystis sp. PCC 6803
DescriptionProtein phosphorylation via serine/threonine protein kinases (Spk) is a widespread mechanism to adjust cellular processes toward changing environmental conditions. To study their role(s) in cyanobacteria, we established a collection of 11 completely segregated spk mutants among the 12 annotated Spk’s in the model cyanobacterium Synechocystis sp. PCC 6803. Screening of the mutant collection revealed that especially the mutant defective in SpkB encoded by slr1697 showed clear deviations compared to wild type (WT) regarding carbon metabolism, i.e., a reduced growth rate at low CO2 and in the presence of glucose, different glycogen accumulation patterns, and a higher tolerance to external H2O2 than the WT. The proteome of ∆spkB showed several distinct differences compared to WT, which indicate changes of the cell surface but also metabolic functions. A phospho-proteome analysis revealed decreased phosphorylation of the carboxysome-associated protein CcmM and the regulatory PII protein in the mutant compared to WT, whereas the allophycocyanin alpha subunit was stronger phosphorylated. The decreased phosphorylation of PII was verified in Western-blot experiments, indicating a clearly delayed PII phosphorylation in cells shifted from nitrate-containing to nitrate-free medium. Furthermore, the mutant ∆spkB showed differences in the state transition consistent with the changed phosphorylation of allophycocyanin. Collectively our results indicate that SpkB is an important regulator under different environmental conditions in Synechocystis and seems to interact in the PII phosphorylation and probably with further substrates in a kinase network.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:27:05.624.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPhilipp Spaet
SpeciesList scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-24 04:29:07ID requested
12024-01-26 09:32:00announced
22024-10-22 06:27:06announced2024-10-22: Updated project metadata.
Publication List
Barske T, Sp, ä, t P, Schubert H, Walke P, Ma, č, ek B, Hagemann M, The Role of Serine/Threonine-Specific Protein Kinases in Cyanobacteria - SpkB Is Involved in Acclimation to Fluctuating Conditions in Synechocystis sp. PCC 6803. Mol Cell Proteomics, 22(11):100656(2023) [pubmed]
10.1016/j.mcpro.2023.100656;
Keyword List
submitter keyword: adaptation,Cyanobacteria, kinases, phosphorylation, Synechocystis sp. PCC6803, SpkB
Contact List
Prof. Dr. Martin Hagemann
contact affiliationInstitut für Biowissenschaften, Abteilung Pflanzenphysiologie, Universität Rostock, A.-Einstein-Str. 3, Rostock D-18059, Germany
contact emailmartin.hagemann@uni-rostock.de
lab head
Philipp Spaet
contact affiliationUniversity Tuebingen Department of Quantitative Proteomics
contact emailphilipp.spaet@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD040383
PRIDE project URI
Repository Record List
[ + ]