PXD040374

PXD040374 is an original dataset announced via ProteomeXchange.

Title	Structural basis of activation and inhibition of the Ca2+/calmodulin-sensitive adenylyl cyclase 8 (XL-MS)
Description	Membrane adenylyl cyclases (ACs) catalyze the conversion of ATP to cAMP, a second messenger involved in different signaling pathways. AC8 is one of the 9 membrane-bound isoforms, present in the brain and implicated in cognitive functions. AC8 is regulated by Ca2+/CaM and different G proteins but structural evidence on its regulation is scarce. We solved the structure of full-length AC8 in complex with Gas and CaM. ACs contain large stretches of disordered/highly flexible domains that cannot be resolved with cryo-EM. To overcome this limitation, we have studied AC8's interaction with CaM, Gas and Gbg using crosslinking mass spectrometry (XL-MS).
HostingRepository	PRIDE
AnnounceDate	2024-02-14
AnnouncementXML	Submission_2024-02-14_06:30:13.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dina Schuster
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-24 02:36:57	ID requested
⏵ 1	2024-02-14 06:30:13	announced

10.1038/S44319-024-00076-Y;

submitter keyword: crosslinking mass spectrometry, XL-MS, membrane protein

Volodymyr M. Korkhov
contact affiliation	Laboratory of Biomolecular Research, Division of Biology and Chemistry, Paul Scherrer Institute, Villigen, Switzerland; Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Switzerland
contact email	volodymyr.korkhov@psi.ch
lab head
Dina Schuster
contact affiliation	ETH Zurich
contact email	dschuster@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040374

PRIDE project URI

• PRIDE
  1. PXD040374
     1. Label: PRIDE project
     2. Name: Structural basis of activation and inhibition of the Ca2+/calmodulin-sensitive adenylyl cyclase 8 (XL-MS)