PXD040373

PXD040373 is an original dataset announced via ProteomeXchange.

Title	Is there difference in proteomic profile of stimulated and un-stimulated saliva samples from pregnant women with/without obesity and periodontitis?
Description	This study aimed to compare the proteomic profile of stimulated and unstimulated saliva sam-ples from pregnant women with/without obesity and periodontitis. Initially, 126 pregnant women were recruited and, after exclusions, were allocated into: with obesity and periodontitis (OP); with obesity but without periodontitis (OWP); with normal BMI but with periodontitis (NP); with normal BMI and without periodontitis (NWP). Stimulated saliva (SS) and unstimulated saliva (US) samples were collected, and salivary proteins were extracted and individually processed by proteomic analysis (nLC-ESI-MS/MS). Proteins involved with immune response process, antiox-idant activity, and retina homeostasis were decreased or absent in SS samples from all groups (i.e., Antileukoproteinase, Lysozyme C, Alpha-2-macroglobulin-like protein 1, Heat shock proteins – 70kDa 1-like, 1A, 1B, 6, Heat shock-related 70 kDa protein 2, Putative Heat shock 70 kDa protein 7, Heat shock cognate 71 kDa). Also, proteins related to carbohydrate metabolic process and gly-colytic and glucose metabolic process were absent in SS, mainly from OP and OWP (i.e., Fru-tose-bisphosphate aldose A, Glusoce-6-phosphate isomerase, Pyruvate kinase). Saliva stimulation decreased important proteins involved with immune response and inflammation process in all groups. Unstimulated salivary samples seem to be the best choice to proteomic approach in pregnant women.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:10:40.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerson Foratori Junior
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2 Q-Tof; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-24 02:04:05	ID requested
1	2023-10-24 12:59:45	announced
⏵ 2	2023-11-14 09:10:41	announced	2023-11-14: Updated project metadata.

Foratori-Junior GA, Ventura TMO, Grizzo LT, Jesuino BG, Castilho AVSS, Buzalaf MAR, Sales-Peres SHC, Is There a Difference in the Proteomic Profile of Stimulated and Unstimulated Saliva Samples from Pregnant Women with/without Obesity and Periodontitis? Cells, 12(10):(2023) [pubmed]

submitter keyword: Obesity, Saliva, Pregnancy, Proteomics, Periodontitis

Gerson Aparecido Foratori Junior
contact affiliation	Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo
contact email	gerson.foratori@usp.br
lab head
Gerson Foratori Junior
contact affiliation	Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo
contact email	gerson.foratori@usp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040373
     1. Label: PRIDE project
     2. Name: Is there difference in proteomic profile of stimulated and un-stimulated saliva samples from pregnant women with/without obesity and periodontitis?