PXD040363

PXD040363 is an original dataset announced via ProteomeXchange.

Title	Muscle-derived extracellular vesicles transport glycolysis enzyme to mediate muscle-to-bone crosstalk
Description	Identification of cues from skeletal muscle that control bone formation is essential for understanding the crosstalk between muscle and bone and for developing novel therapeutics for degenerative bone diseases. Here, we identified that skeletal muscle secreted abundant extracellular vesicles (Mu-EVs). Mu-EVs traveled via blood vessels to bone and were phagocytized by bone marrow mesenchymal stem/stromal cells (BMSCs). Mu-EVs promoted osteogenic differentiation of BMSCs and protected mice against disuse osteoporosis. The quantity and bioactivity of Mu-EVs were tightly correlated with the function of skeletal muscle. Protein mass spectrometry revealed dozens of proteins in Mu-EVs potentially involved in bone metabolism regulation, especially glycolysis. Further analysis indicated that Mu-EVs promoted the glycolysis of BMSCs by delivering LDHA into BMSCs to promote glycolysis. Taken together, these findings uncovered that Mu-EVs function as a non-canonical myokine to regulate BMSC metabolism and bone formation. Supplementation of normal Mu-EVs is a potential therapy for disuse osteoporosis.
HostingRepository	PRIDE
AnnounceDate	2026-08-19
AnnouncementXML	Submission_2026-08-19_06:17:32.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shixing Ma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-23 06:18:46	ID requested
⏵ 1	2026-08-19 06:17:33	announced

10.1016/J.CMET.2023.10.013;

submitter keyword: Skeletal muscle;Extracellular vesicles;Glycolysis

Weidong Tian
contact affiliation	State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Engineering Research Center of Oral Translational Medicine, Ministry of Education & National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
contact email	drtwd@sina.com
lab head
Shixing Ma
contact affiliation	West China Hospital of Stomatology, Sichuan University; State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Oral Regenerative Medicine.
contact email	1208017287@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD040363

PRIDE project URI

• PRIDE
  1. PXD040363
     1. Label: PRIDE project
     2. Name: Muscle-derived extracellular vesicles transport glycolysis enzyme to mediate muscle-to-bone crosstalk