PXD040354

PXD040354 is an original dataset announced via ProteomeXchange.

Title	Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors
Description	Protein prenylation is one example of a broad class of post-translational modifications where proteins are covalently linked to various hydrophobic moieties. To globally identify and monitor levels of all prenylated proteins in a cell simultaneously, our laboratory and others have developed chemical proteomic approaches that rely on the metabolic incorporation of isoprenoid analogues bearing bio-orthogonal functionality followed by enrichment and subsequent quantitative proteomic analysis. Here, we report on several improvements in the synthesis of the alkyne-containing isoprenoid analogue and the application of that probe study the prenylomes of motor neurons, astrocytes and their stem cell progenitors. The identification of numerous prenylated proteins in various ES-derived primary cells including 82 different proteins in ES astrocytes highlights the ability of this method to track the prenylation state of a diverse range of proteins. This strategy should be useful for clarifying how inhibitors of protein prenylation can serve as potent neurite-outgrowth-promoting agents.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:40:01.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shelby Auger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	farnesylated residue; biotinylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-23 03:51:28	ID requested
1	2024-05-21 08:51:08	announced
⏵ 2	2024-10-22 06:40:02	announced	2024-10-22: Updated project metadata.

10.1016/j.bioorg.2024.107365;

Suazo KF, Mishra V, Maity S, Auger SA, Justyna K, Petre AM, Ottoboni L, Ongaro J, Corti SP, Lotti F, Przedborski S, Distefano MD, Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors. Bioorg Chem, 147():107365(2024) [pubmed]

submitter keyword: Prenylation, Astrocytes, ALS, Chemical enrichment, Neurons

Mark D Distefano
contact affiliation	Chemistry Department, University of Minnesota
contact email	diste001@umn.edu
lab head
Shelby Auger
contact affiliation	University of Minnesota
contact email	auger054@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040354

PRIDE project URI

• PRIDE
  1. PXD040354
     1. Label: PRIDE project
     2. Name: Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors