PXD040353

PXD040353 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	O-GlcNAcylation and chaperonin CCT5 protect OTX2 from aggregation in medulloblastoma cellsO-GlcNAcylation andanalysis of OTX2 O-GlcNac sites and dependent interactions
Description	In this study, we demonstrated that OTX2 was transcriptionally and post-transcriptionally regulated by O-GlcNAcylation. We describe the modes of degradation of this essential homeobox protein involving both the proteasome and macroautophagy. While the proteasome remains the canonical degradation pathway, we demonstrated that OTX2 forms oligomers and/or aggregates that are resistant to proteasomal degradation and require macroautophagy. Furthermore, elevated cellular concentration of OTX2 promoted aggregation, OTX2 could either interact with the chaperonin CCT5 or be O-GlcNAcylated at the key residues (S1135/S136/T137) to prevent its oligomerization, suggesting a protective role of O-GlcNAcylation in OTX2 turnover.
HostingRepository	PRIDE
AnnounceDate	2024-01-22
AnnouncementXML	Submission_2024-01-22_15:52:14.374.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040353
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stephanie Olivier-Van Stichelen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	O-(N-acetylamino)galactosyl-L-threonine; O-(N-acetylamino)galactosyl-L-serine
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-23 03:51:07	ID requested
⏵ 1	2024-01-22 15:52:15	announced

Publication List

10.1016/j.isci.2023.108184;
10.6019/PXD040353;
Wulff-Fuentes E, Boakye J, Kroenke K, Berendt RR, Martinez-Morant C, Pereckas M, Hanover JA, Olivier-Van Stichelen S, -GlcNAcylation regulates OTX2's proteostasis. iScience, 26(11):108184(2023) [pubmed]

10.1016/j.isci.2023.108184;

10.6019/PXD040353;

Wulff-Fuentes E, Boakye J, Kroenke K, Berendt RR, Martinez-Morant C, Pereckas M, Hanover JA, Olivier-Van Stichelen S, -GlcNAcylation regulates OTX2's proteostasis. iScience, 26(11):108184(2023) [pubmed]

Keyword List

submitter keyword: OTX2,EtHCD-MS, O-GlcNAc, interactome

submitter keyword: OTX2,EtHCD-MS, O-GlcNAc, interactome

Contact List

Stephanie Olivier-Van Stichelen
contact affiliation	Medical College of Wisconsin
contact email	solivier@mcw.edu
lab head
Stephanie Olivier-Van Stichelen
contact affiliation	Medical College of Wisconsin
contact email	solivier@mcw.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD040353
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD040353

PRIDE project URI

Repository Record List

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