PXD040339

PXD040339 is an original dataset announced via ProteomeXchange.

Title	Deletion of GSE1 and HDAC1 inactivation alters proteomic profiles and cell cycle control
Description	The DNA damage response (DDR) is orchestrated by multiple, tightly regulated signalling events. We discovered that genetic suppressor element 1 (GSE1) forms a complex with the histone deacetylation 1 (HDAC1) / CoREST complex and that loss of GSE1 hampers DDR induction. To investigate how GSE1 and HDAC1 can regulate DDR and whether their mode of action might be overlapping, we performed a proteomic analysis using quantitative mass spectrometry based on the TMTpro 16-plex isobaric labelling technology (Li et al., 2020). We determined global acetylome and phosphorylome profiles in the nearly haploid human cell line HAP1 (Andersson et al, 1987) using WT, GSE1 KO and HDAC1 CI backgrounds and compared patterns between untreated and etoposide-treated (0.1 µM, 6 hrs) cells.
HostingRepository	PRIDE
AnnounceDate	2024-04-02
AnnouncementXML	Submission_2024-04-02_06:44:42.292.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wolfgang Reiter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-22 10:20:12	ID requested
1	2023-10-23 05:44:55	announced
2	2023-11-14 08:18:34	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-04-02 06:44:43	announced	2024-04-02: Updated project metadata.

10.1093/NAR/GKAD911;

10.1093/nar/gkad911;

Vcelkova T, Reiter W, Zylka M, Hollenstein DM, Schuckert S, Hartl M, Seiser C, GSE1 links the HDAC1/CoREST co-repressor complex to DNA damage. Nucleic Acids Res, 51(21):11748-11769(2023) [pubmed]

submitter keyword: acetylome,HDAC1, GSE1, phosphorylome, HAP1, DNA damage response

Markus Hartl
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	markus.hartl@univie.ac.at
lab head
Wolfgang Reiter
contact affiliation	Mass Spectrometry Facility, Max Perutz Labs Vienna, University of Vienna, Vienna Biocenter, Dr.-Bohr-Gasse 7, 1030 Vienna
contact email	wolfgang.l.reiter@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040339
     1. Label: PRIDE project
     2. Name: Deletion of GSE1 and HDAC1 inactivation alters proteomic profiles and cell cycle control