PXD040335

PXD040335 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Pyruvate kinase M2 modification by a lipid peroxidation byproduct acrolein contributes to kidney fibrosis
Description	This study used a proteomic approach with an anti-Acr-PC antibody followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis to identify several acrolein-modified protein targets. Among these protein targets, pyruvate kinase M2 (PKM2) was found to be modified by acrolein at Cys358, leading to the inactivation of PKM2 contributing to the pathogenesis of renal fibrosis through HIF1 accumulation, aberrant glycolysis, and upregulation of EMT in HFD-STZ-induced DN mice. Finally, PKM2 activity and renal fibrosis in DN mice can be reduced by acrolein scavengers such as hydralazine and carnosine. These results imply that acrolein-modified PKM2 contributes to renal fibrosis in the pathogenesis of DN.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:50.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hsiang-Tsui Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-22 10:18:43	ID requested
1	2023-05-10 13:39:24	announced
⏵ 2	2023-11-14 08:46:56	announced	2023-11-14: Updated project metadata.

Publication List

Kuo CW, Chen DH, Tsai MT, Lin CC, Cheng HW, Tsay YG, Wang HT, Pyruvate kinase M2 modification by a lipid peroxidation byproduct acrolein contributes to kidney fibrosis. Front Med (Lausanne), 10():1151359(2023) [pubmed]

Kuo CW, Chen DH, Tsai MT, Lin CC, Cheng HW, Tsay YG, Wang HT, Pyruvate kinase M2 modification by a lipid peroxidation byproduct acrolein contributes to kidney fibrosis. Front Med (Lausanne), 10():1151359(2023) [pubmed]

Keyword List

submitter keyword: Renal fibrosis
Acrolein
Pyruvate kinase M2
hypoxia-inducible factor 1-alpha
Hydralazine
Carnosine.

submitter keyword: Renal fibrosis

Acrolein

Pyruvate kinase M2

hypoxia-inducible factor 1-alpha

Hydralazine

Carnosine.

Contact List

Hsiang-Tsui Wang
contact affiliation	Department of Pharmacology, National Yang Ming Chiao Tung University
contact email	htwang01@nycu.edu.tw
lab head
Hsiang-Tsui Wang
contact affiliation	National Yang Ming Chiao Tung University
contact email	htwang01@nycu.edu.tw
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD040335
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD040335

PRIDE project URI

Repository Record List

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