PXD040334

PXD040334 is an original dataset announced via ProteomeXchange.

Title	Exploring the selectivity and engineering potential of an NRPS condensation domain involved in the biosynthesis of the thermophilic siderophore fuscachelin
Description	Condensation (C) domains are the key component linking different monomers together, typically forming peptide bonds and occasionally ester bonds, during the nonribosomal peptide synthetase (NRPS). While A domains have been well characterised due to their role in selectivity of the monomers and functioning as a gate keeper in the NRPS biosynthesis, C domains have been a subject of debate as they have not demonstrated signs of “A-domain like” side chain selectivity of its acceptor side. Here, we report our biochemical and structural characterisation of the selectivity of the fuscachelin C3-domain showing that it is not broadly flexible for monomers at the acceptor site, suggesting the need to consider C-domain mutation regarding future NRPS engineering.
HostingRepository	PRIDE
AnnounceDate	2023-06-02
AnnouncementXML	Submission_2023-06-02_01:36:23.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JoelSteele
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Fusion; Bruker Daltonics micrOTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-22 10:18:22	ID requested
⏵ 1	2023-06-02 01:36:23	announced

Dataset with its publication pending

submitter keyword: Nonribosomal peptide

Biosynthesis

Condensation domain

Peptidyl carrier protein

Biocatalysis

Depsipeptide

MaxCryle
contact affiliation	(Monash University). Y. T. Candace Ho: yee.ho@monash.edu (Monash University)
contact email	max.cryle@monash.edu
lab head
JoelSteele
contact affiliation	Monash
contact email	joel.steele@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD040334

PRIDE project URI

• PRIDE
  1. PXD040334
     1. Label: PRIDE project
     2. Name: Exploring the selectivity and engineering potential of an NRPS condensation domain involved in the biosynthesis of the thermophilic siderophore fuscachelin