PXD040323

PXD040323 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TOC1 and PRR5 as regulators of temperature compensation in the Arabidopsis circadian clock (12˚C vs 22˚C)
Description	Ambient temperature potentially affects all biological systems. However, the period length of circadian clocks is close to 24 h over a broad range of physiological temperatures, known as temperature compensation. The mechanism underpinning the temperature compensation remains largely unknown. Here, we show that inhibitors of time progression, TIMING OF CAB EXPRESSION 1 (TOC1) and PSEUDO-RESPONSE REGULATOR 5 (PRR5) proteins, are required for the temperature compensation in Arabidopsis thaliana (Arabidopsis). Compared to the wild-type, the prr5 toc1 double mutant shows a short period at high temperatures but not low temperatures. TOC1 and PRR5 proteins are ubiquitinated and degraded at low temperatures, by a ubiquitin E3 ligase LOV KELCH PROTEIN 2 (LKP2) that was sought as a minor player in the clock. The lkp2 mutants show long periods only at lower temperatures. Collectively, the clock keeps its periodicity against ambient temperature by the temperature-dependent quantity control of inhibitors of time progression.
HostingRepository	jPOST
AnnounceDate	2025-02-22
AnnouncementXML	Submission_2025-02-21_07:00:07.842.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040323
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Norihito Nakamichi
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino acetylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-22 02:06:37	ID requested
⏵ 1	2025-02-21 07:00:08	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: PRR5, TOC1, Arabidopsis, circadian clock, temperature

submitter keyword: PRR5, TOC1, Arabidopsis, circadian clock, temperature

Contact List

Norihito Nakamichi
lab head
Norihito Nakamichi
contact affiliation	Nagoya University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002056/