PXD040318

PXD040318 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporally direct capturing global substrates of post-translational modifying enzymes in living cells
Description	Methods for direct capturing global substrates of protein-modifying enzymes in living cells are with many challenges, and yet largely unexplored. Here, we report a strategy to directly capture substrates of protein-modifying enzymes via PTM-acceptor residue crosslinking in living cells, enabling global profiling of substrates of PTM-enzymes and validation of PTM-sites in a straightforward manner.
HostingRepository	iProX
AnnounceDate	2023-02-22
AnnouncementXML	Submission_2024-02-28_18:53:43.924.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Linhui Zhai
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-21 22:06:04	ID requested
1	2024-01-29 23:18:13	announced
⏵ 2	2024-02-28 18:53:44	announced	2024-02-29: Update information.

Hu H, Hu W, Guo AD, Zhai L, Ma S, Nie HJ, Zhou BS, Liu T, Jia X, Liu X, Yao X, Tan M, Chen XH, Spatiotemporal and direct capturing global substrates of lysine-modifying enzymes in living cells. Nat Commun, 15(1):1465(2024) [pubmed]

submitter keyword: post-translational modification, living cell, crosslinking

Minjia Tan
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	mjtan@simm.ac.cn
lab head
Linhui Zhai
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	zhailinhui@simm.ac.cn
dataset submitter

iProX dataset URI