PXD040292

PXD040292 is an original dataset announced via ProteomeXchange.

Title	New insights on sperm function in male infertility of unknown origin: proteomics characterization
Description	The global trend on (male)infertility is concerning and the unidentifiable causes in half of the cases demands a better understanding of the molecular, biochemical and metabolic mechanisms as well as the identification of external and internal factors operating behind it, that might help to explain this apparently unjustified infertility. This can only be achieved through a comprehensive analysis of the infertile men, in which external (lifestyle, occupational and environmental factors) and internal factors (psychological distress) should also be evaluated and considered, but also assessing sperm function beyond the routine seminal analysis. In this prospective cohort study, 79 sperm samples, from men who were male partners in couples seeking for infertility treatment, were collected at the Reproductive Medicine Unit (CHUC) from July 2018 to July 2022 and analyzed by SWATH-MS. Based on couples’ clinical data, seminal/hormonal analysis, and strict exclusion criteria, samples were categorized in the different groups, namely control (CTRL; 50 individuals), idiopathic infertile (ID; 19 patients) and unexplained infertile (UMI; 10 patients) men. In general, ID patients presented the worst sperm functional profile, while the UMI patients were observed to be similar to controls. These differences were also observed at the proteomics levels, which revealed 145 differentially expressed proteins (DEP) between the three groups, with more than 120 proteins being altered between ID and the other groups, and only 14 proteins were considered altered between CTRL and UMI.
HostingRepository	PRIDE
AnnounceDate	2024-02-28
AnnouncementXML	Submission_2024-02-28_09:45:51.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Anjo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-20 08:29:50	ID requested
⏵ 1	2024-02-28 09:45:52	announced

Pacheco RI, Cristo MI, Anjo SI, Silva AF, Sousa MI, Tavares RS, Sousa AP, Almeida Santos T, Moura-Ramos M, Caramelo F, Manadas B, Ramalho-Santos J, Amaral SG, New Insights on Sperm Function in Male Infertility of Unknown Origin: A Multimodal Approach. Biomolecules, 13(10):(2023) [pubmed]

10.3390/biom13101462;

submitter keyword: xxxxxxIdiopathic male infertility

Unexplained male infertility

sperm function

sperm proteomics

Sandra Amaral
contact affiliation	​CNC - Center for Neuroscience and Cell Biology
contact email	scgamaral@gmail.com
lab head
Sandra Anjo
contact affiliation	​Center for Neuroscience and Cell Biology – University of Coimbra
contact email	sandra.isabel.anjo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040292

PRIDE project URI

• PRIDE
  1. PXD040292
     1. Label: PRIDE project
     2. Name: New insights on sperm function in male infertility of unknown origin: proteomics characterization