PXD040286 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Atmospheric hydrogen oxidation extends to the domain archaea |
Description | Diverse aerobic bacteria use atmospheric hydrogen (H2) and carbon monoxide (CO) as energy sources to support growth and survival. Though recently discovered, trace gas oxidation is now recognised as a globally significant process that serves as the main sink in the biogeochemical H2 cycle and sustains microbial biodiversity in oligotrophic ecosystems. While trace gas oxidation has been reported in nine phyla of bacteria, it was not known whether archaea also use atmospheric H2. Here we show that a thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes H2 and CO to sub-atmospheric levels. Oxidation occurred during both growth and survival across a wide range of temperatures (10 to 70°C). Genomic analysis demonstrated that A. brierleyi encodes a canonical carbon monoxide dehydrogenase and, unexpectedly, four distinct [NiFe]-hydrogenases from subgroups not known to mediate aerobic H2 uptake. Quantitative proteomic analyses showed that A. brierleyi differentially produced these enzymes in response to electron donor and acceptor availability. A previously unidentified group 1 [NiFe]-hydrogenase, with a unique genetic arrangement, is constitutively expressed and upregulated during stationary phase and aerobic hydrogenotrophic growth. Another archaeon, Metallosphaera sedula, was also found to oxidize atmospheric H2. These results suggest that trace gas oxidation is a common trait of aerobic archaea, which likely plays a role in their survival and niche expansion, including during dispersal through temperate environments. These findings also demonstrate that atmospheric H2 consumption is a cross-domain phenomenon, suggesting an ancient origin of this trait, and identify previously unknown microbial and enzymatic sinks of atmospheric H2 and CO. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-26 |
AnnouncementXML | Submission_2024-02-26_01:56:50.159.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joel Steele |
SpeciesList | scientific name: Acidianus brierleyi; NCBI TaxID: 41673; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-20 07:13:31 | ID requested | |
⏵ 1 | 2024-02-26 01:56:50 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Hydrogen metabolism, Archaea,Microbiology, Archaeal physiology, Trace gas oxidation, Acidianus, Environmental microbiology |
Contact List
Chris Greening |
contact affiliation | Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. Pok Man Leung, pok.leung@monash.edu, Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia Ralf B. Schittenhelm, ralf.schittenhelm@monash.edu, Monash Proteomics and Metabolomics Facility and Department of Biochemistry, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia |
contact email | chris.greening@monash.edu |
lab head | |
Joel Steele |
contact affiliation | Monash |
contact email | joel.steele@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040286
- Label: PRIDE project
- Name: Atmospheric hydrogen oxidation extends to the domain archaea