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PXD040286

PXD040286 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAtmospheric hydrogen oxidation extends to the domain archaea
DescriptionDiverse aerobic bacteria use atmospheric hydrogen (H2) and carbon monoxide (CO) as energy sources to support growth and survival. Though recently discovered, trace gas oxidation is now recognised as a globally significant process that serves as the main sink in the biogeochemical H2 cycle and sustains microbial biodiversity in oligotrophic ecosystems. While trace gas oxidation has been reported in nine phyla of bacteria, it was not known whether archaea also use atmospheric H2. Here we show that a thermoacidophilic archaeon, Acidianus brierleyi (Thermoproteota), constitutively consumes H2 and CO to sub-atmospheric levels. Oxidation occurred during both growth and survival across a wide range of temperatures (10 to 70°C). Genomic analysis demonstrated that A. brierleyi encodes a canonical carbon monoxide dehydrogenase and, unexpectedly, four distinct [NiFe]-hydrogenases from subgroups not known to mediate aerobic H2 uptake. Quantitative proteomic analyses showed that A. brierleyi differentially produced these enzymes in response to electron donor and acceptor availability. A previously unidentified group 1 [NiFe]-hydrogenase, with a unique genetic arrangement, is constitutively expressed and upregulated during stationary phase and aerobic hydrogenotrophic growth. Another archaeon, Metallosphaera sedula, was also found to oxidize atmospheric H2. These results suggest that trace gas oxidation is a common trait of aerobic archaea, which likely plays a role in their survival and niche expansion, including during dispersal through temperate environments. These findings also demonstrate that atmospheric H2 consumption is a cross-domain phenomenon, suggesting an ancient origin of this trait, and identify previously unknown microbial and enzymatic sinks of atmospheric H2 and CO.
HostingRepositoryPRIDE
AnnounceDate2024-02-26
AnnouncementXMLSubmission_2024-02-26_01:56:50.159.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJoel Steele
SpeciesList scientific name: Acidianus brierleyi; NCBI TaxID: 41673;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-20 07:13:31ID requested
12024-02-26 01:56:50announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Hydrogen metabolism, Archaea,Microbiology, Archaeal physiology, Trace gas oxidation, Acidianus, Environmental microbiology
Contact List
Chris Greening
contact affiliationDepartment of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. Pok Man Leung, pok.leung@monash.edu, Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia Ralf B. Schittenhelm, ralf.schittenhelm@monash.edu, Monash Proteomics and Metabolomics Facility and Department of Biochemistry, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia
contact emailchris.greening@monash.edu
lab head
Joel Steele
contact affiliationMonash
contact emailjoel.steele@monash.edu
dataset submitter
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Dataset FTP location
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