PXD040284 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays |
Description | Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for the identification of protein-ligand interactions. Here, we have investigated eight combinations of several recently introduced MS-based advancements, including the Phase-Constrained Spectral Deconvolution Method, Field Asymmetric Ion Mobility Spectrometry, and the implementation of a carrier sample as improved MS-based acquisition approaches for thermal stability assays (iMAATSA). We used intact Jurkat cells treated with a commercially available MEK inhibitor, followed by heat treatment, to prepare a set of unfractionated isobarically-labeled proof-of-concept samples to compare the performance of eight different iMAATSAs. Finally, the best-performing iMAATSA was compared to a conventional approach and evaluated in a fractionation experiment. Improvements of up to 82% and 86% were demonstrated in protein identifications and high-quality melting curves, respectively, over the conventional approach in the proof-of-concept study, while an approximately 12% improvement in melting curve comparisons was achieved in the fractionation experiment. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:47:44.751.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Clifford Phaneuf |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-20 07:12:48 | ID requested | |
1 | 2023-05-10 14:44:39 | announced | |
⏵ 2 | 2023-11-14 08:47:47 | announced | 2023-11-14: Updated project metadata. |
Publication List
Phaneuf CG, Aizikov K, Grinfeld D, Kreutzmann A, Mourad D, Lange O, Dai D, Zhang B, Belenky A, Makarov AA, Ivanov AR, Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays. Commun Chem, 6(1):64(2023) [pubmed] |
Keyword List
submitter keyword: CETSA, mass spectrometry, TPP, target identification, thermal stability assays, drug-protein interactions |
Contact List
Dr. Alexander R. Ivanov |
contact affiliation | Northeastern University, 360 Huntington Ave., Boston, Massachusetts 02115, United StatesBarnett Institute of Chemical and Biological Analysis, Department of Chemistry and Chemical Biology, |
contact email | a.ivanov@northeastern.edu |
lab head | |
Clifford Phaneuf |
contact affiliation | sanofi |
contact email | clifford.phaneuf@sanofi.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040284
- Label: PRIDE project
- Name: Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays