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PXD040274

PXD040274 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes
DescriptionThe larval stage of the cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a lethal disease if untreated. To date, drug treatment options are limited, non-curative and need improvement. In this project we investigated the proteomic composition of in vitro generated metacestodes that are the disease-causing stage of the parasite. We analysed the composition of the vesicle tissue (VT), the vesicle fluid (VF), as well as the changes in the surrounding culture medium (CM). Further we compared the composition of VF ex vivo material from infected mice. As antigen B (AgB) subunits were the most prominent proteins in both VFs and CM, we followed up on these specific proteins and assessed the possibility of re-uptake of AgB into the metacestode, by targeted proteomics against HA-tagged AgB. Six proteomic analyses were performed: 1_ VF and VT 2_ CM (in vitro samples) 3_ VF ex vivo samples from infected mice 4_ HA-AgB8 calibration (in vitro samples) 5_ HA-AgB8/1 uptake (in vitro samples) 6_ HA-AgB8/2 uptake (in vitro samples)
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:50:57.229.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterManfred Heller
SpeciesList scientific name: Echinococcus multilocularis; NCBI TaxID: 6211;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-20 05:25:32ID requested
12024-07-19 07:41:53announced
22024-10-22 06:50:58announced2024-10-22: Updated project metadata.
Publication List
M, ü, ller J, Preza M, Kaethner M, Rufener R, Braga S, Uldry AC, Heller M, Lundstr, ö, m-Stadelmann B, metacestodes. Front Cell Infect Microbiol, 13():1170763(2023) [pubmed]
10.3389/fcimb.2023.1170763;
Keyword List
submitter keyword: model system, host-parasite interplay, transport,alveolar echinococcosis, untargeted proteomics, cestodes, targeted proteomics
Contact List
Britta Lundström-Stadelmann
contact affiliationInstitute of Parasitology, University of Bern, Switzerland
contact emailbritta.lundstroem@unibe.ch
lab head
Manfred Heller
contact affiliationProteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact emailpmscf@dbmr.unibe.ch
dataset submitter
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