PXD040274 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes |
Description | The larval stage of the cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a lethal disease if untreated. To date, drug treatment options are limited, non-curative and need improvement. In this project we investigated the proteomic composition of in vitro generated metacestodes that are the disease-causing stage of the parasite. We analysed the composition of the vesicle tissue (VT), the vesicle fluid (VF), as well as the changes in the surrounding culture medium (CM). Further we compared the composition of VF ex vivo material from infected mice. As antigen B (AgB) subunits were the most prominent proteins in both VFs and CM, we followed up on these specific proteins and assessed the possibility of re-uptake of AgB into the metacestode, by targeted proteomics against HA-tagged AgB.
Six proteomic analyses were performed:
1_ VF and VT
2_ CM (in vitro samples)
3_ VF ex vivo samples from infected mice
4_ HA-AgB8 calibration (in vitro samples)
5_ HA-AgB8/1 uptake (in vitro samples)
6_ HA-AgB8/2 uptake (in vitro samples) |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:50:57.229.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manfred Heller |
SpeciesList | scientific name: Echinococcus multilocularis; NCBI TaxID: 6211; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-20 05:25:32 | ID requested | |
1 | 2024-07-19 07:41:53 | announced | |
⏵ 2 | 2024-10-22 06:50:58 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: model system, host-parasite interplay, transport,alveolar echinococcosis, untargeted proteomics, cestodes, targeted proteomics |
Contact List
Britta Lundström-Stadelmann |
contact affiliation | Institute of Parasitology, University of Bern, Switzerland |
contact email | britta.lundstroem@unibe.ch |
lab head | |
Manfred Heller |
contact affiliation | Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland |
contact email | pmscf@dbmr.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040274
- Label: PRIDE project
- Name: Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes