PXD040274

PXD040274 is an original dataset announced via ProteomeXchange.

Title	Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes
Description	The larval stage of the cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a lethal disease if untreated. To date, drug treatment options are limited, non-curative and need improvement. In this project we investigated the proteomic composition of in vitro generated metacestodes that are the disease-causing stage of the parasite. We analysed the composition of the vesicle tissue (VT), the vesicle fluid (VF), as well as the changes in the surrounding culture medium (CM). Further we compared the composition of VF ex vivo material from infected mice. As antigen B (AgB) subunits were the most prominent proteins in both VFs and CM, we followed up on these specific proteins and assessed the possibility of re-uptake of AgB into the metacestode, by targeted proteomics against HA-tagged AgB. Six proteomic analyses were performed: 1_ VF and VT 2_ CM (in vitro samples) 3_ VF ex vivo samples from infected mice 4_ HA-AgB8 calibration (in vitro samples) 5_ HA-AgB8/1 uptake (in vitro samples) 6_ HA-AgB8/2 uptake (in vitro samples)
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:50:57.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Echinococcus multilocularis; NCBI TaxID: 6211;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-20 05:25:32	ID requested
1	2024-07-19 07:41:53	announced
⏵ 2	2024-10-22 06:50:58	announced	2024-10-22: Updated project metadata.

M, ü, ller J, Preza M, Kaethner M, Rufener R, Braga S, Uldry AC, Heller M, Lundstr, ö, m-Stadelmann B, metacestodes. Front Cell Infect Microbiol, 13():1170763(2023) [pubmed]

10.3389/fcimb.2023.1170763;

submitter keyword: model system, host-parasite interplay, transport,alveolar echinococcosis, untargeted proteomics, cestodes, targeted proteomics

Britta Lundström-Stadelmann
contact affiliation	Institute of Parasitology, University of Bern, Switzerland
contact email	britta.lundstroem@unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040274

PRIDE project URI

• PRIDE
  1. PXD040274
     1. Label: PRIDE project
     2. Name: Targeted and non-targeted proteomics to characterize the parasite proteins of Echinococcus multilocularis metacestodes