PXD040257

PXD040257 is an original dataset announced via ProteomeXchange.

Title	A chaperone-like function of FUS ensures TAZ condensates dynamics and transcriptional activation
Description	The Hippo pathway plays important roles in organ development, tissue homeostasis, and tumor growth, and its downstream effector TAZ is a transcriptional coactivator that promotes target gene expression through the formation of biomolecular condensates. However, the mechanisms that regulate the biophysical properties of TAZ condensates to enable Hippo signaling are not well understood. Here, using chemical cross-linking combined with an unbiased proteomic approach, we show that FUS associates with TAZ condensates and exerts a chaperone-like effect to maintain their proper liquidity and robust- transcriptional activity. Mechanistically, the low complexity sequence domain of FUS targets the coiled-coil domain of TAZ in a phosphorylation-regulated manner, which ensures the liquidity and dynamicity of TAZ condensates. In cells lacking FUS, TAZ condensates transition into gel/solid-like assembles with immobilized TAZ, leading to reduced expression of target genes and inhibition of pro-tumorigenic activity. Thus, our findings identify a chaperone-like function of FUS in Hippo regulation and demonstrate that appropriate biophysical properties of transcriptional condensates are essential for gene activation.
HostingRepository	iProX
AnnounceDate	2023-02-17
AnnouncementXML	Submission_2024-06-06_01:13:19.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huasong Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-19 19:43:46	ID requested
1	2023-11-05 00:41:42	announced
⏵ 2	2024-06-06 01:13:20	announced	2024-06-06: Update information.

Shao Y, Shu X, Lu Y, Zhu W, Li R, Fu H, Li C, Sun W, Li Z, Zhang Y, Cao X, Ye X, Ajiboye E, Zhao B, Zhang L, Wu H, Feng XH, Yang B, Lu H, A chaperone-like function of FUS ensures TAZ condensate dynamics and transcriptional activation. Nat Cell Biol, 26(1):86-99(2024) [pubmed]

submitter keyword: XL-MS, IP-MS

Huasong Lu
contact affiliation	Zhejiang University
contact email	huasong_lu@zju.edu.cn
lab head
Huasong Lu
contact affiliation	Zhejiang University
contact email	huasong_lu@zju.edu.cn
dataset submitter

iProX dataset URI