PXD040245

PXD040245 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TMT labeling and LC-MS/MS strategy to quantify differentially abundant proteins among COVID-19 patients
Description	Coronavirus Disease 2019 (COVID-19) is characterized by a dysregulated host response. Proteomic studies have shed light in its complex pathogenesis, but there are few studies using cells, such as peripheral blood mononuclear cells (PBMCs). Here, we used TMT-based quantitative proteomics to characterize PBMCs from COVID-19 patients along their clinical course. Blood samples were collected prospectively from 29 patients with COVID-19 (confirmed by PCR in a nasopharynx swab) and 11 healthy volunteers (HVs), between May and September 2020. Samples were obtained at the first day of admission (D0; n=30), after seven days of hospitalization (D7; n=17), and 31 days after hospital discharge (Convalescent Sample; CS30; n=15). Protein extraction, quantification, reducing/alkylating, methanol/chloroform precipitations, digestion, and TMT Labeling were performed in PBM. The mass spectrometric data were acquired in a Orbitrap Fusion Lumos and data analysis was performed in Proteome Discoverer and imported to the R software environment for further analysis.
HostingRepository	MassIVE
AnnounceDate	2023-08-22
AnnouncementXML	Submission_2023-08-22_01:17:29.383.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe G F Leite
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	TMT6plex
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-17 12:18:23	ID requested
⏵ 1	2023-08-22 01:17:29	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: PBMC, COVID-19, TMT, SARS-CoV-2

submitter keyword: PBMC, COVID-19, TMT, SARS-CoV-2

Contact List

Reinaldo Salomao
contact affiliation	Escola Paulista de Medicina/UNIFESP
contact email	rsalomao@unifesp.br
lab head
Giuseppe Leite
contact affiliation	Escola Paulista de Medicina/UNIFESP
contact email	giuseppe.gianini@unifesp.br
lab head
Giuseppe G F Leite
contact affiliation	Unifesp
contact email	giuseppe.gianini@unifesp.br
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091319/