PXD040240 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics Analysis of Lymphoblastoid Cell lines from Patients with Amyotrophic Lateral Sclerosis |
| Description | Amyotrophic lateral sclerosis (ALS) consists in the progressive degeneration of motor neurons, caused by mechanisms that are poorly understood and for which there is no cure. Some of the cellular perturbations associated with ALS can be detected in peripheral cells, including lym-phocytes from blood. A related cell system that is very suitable for research consists in human lymphoblastoid cell lines (LCLs), which are immortalized lymphocytes. LCLs that can be easily expanded in culture and can be maintained for long periods of time as stable cultures. We inves-tigated, on a small set of LCLs, if proteomics analysis by liquid chromatography followed by tandem mass spectrometry reveals proteins that are differentially present in ALS versus healthy controls. We found that individual proteins and cellular and molecular pathways in which these proteins participate, are detected as differentially present in the ALS samples. Some of these proteins and pathways are already known to be perturbed in ALS, while others are new and present interest for further investigations. These observations suggest that more detailed pro-teomics analysis of LCLs, using a larger number of samples, represents a promising approach for investigating ALS mechanisms and to search for therapeutic agents. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_08:44:14.585.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Danielle Whitham |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Xevo G2-XS QTof |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-02-17 08:28:18 | ID requested | |
| 1 | 2023-03-12 09:49:55 | announced | |
| ⏵ 2 | 2023-11-14 08:44:17 | announced | 2023-11-14: Updated project metadata. |
Publication List
| Whitham D, Belenkiy E, Darie CC, Radu A, Proteomics Analysis of Lymphoblastoid Cell Lines from Patients with Amyotrophic Lateral Sclerosis. Molecules, 28(5):(2023) [pubmed] |
Keyword List
| submitter keyword: Amyotopphic lateral sclerosis |
| proteomics |
| mass spectrometry |
| biomarkers |
Contact List
| Costel C. Darie |
|---|
| contact affiliation | Biochemistry and Proteomics Laboratories, Deparment of Chemistry and biomolecular Science, Clarkson University, Potsdam NY, USA |
| contact email | cdarie@clarkson.edu |
| lab head | |
| Danielle Whitham |
|---|
| contact affiliation | Clarkson University |
| contact email | whithad@clarkson.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040240 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040240
- Label: PRIDE project
- Name: Proteomics Analysis of Lymphoblastoid Cell lines from Patients with Amyotrophic Lateral Sclerosis
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