PXD040237
PXD040237 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Direct phosphorylation of SIZ1 by SnRK2s kinase to response the osmotic stress in Arabidopsis |
| Description | SUMOylation is an essential and highly dynamic post-translational modification that regulates developmental processes and stress adaptations in plants to environmental cues. The global SUMOylation is quickly induced by dehydration and hyperosmotic stresses in plants, while the detailed mechanism underlying such SUMOylation dynamics is largely unknown. Herein, we report key components in osmotic stress and abscisic acid signaling, SNF1-related protein kinase 2s (SnRK2s), phosphorylate SUMO E3 ligase SAP and MIZ1-1 (SIZ1) to stabilize SIZ1 protein under stress conditions. The Ser820 residue is a functional SnRK2 phosphosite and non-phosphorylatable SIZ1S820A is unstable in vivo and in vitro. Under osmotic stress, the rapidly activated protein kinases SnRK2.4 and SnRK2.6 can phosphorylate SIZ1, which enhances the stability of SIZ1, and participates in the response to osmotic stress. However, the instability of SIZ1S820A protein was independent of COP1, indicating that there might be a new degradation mechanism for SIZ1. Our studies suggest that SnRK2s induced phosphorylation regulates SIZ1 stability to precisely modulates SUMOylation dynamics upon osmotic stresses. Additionally, such a reciprocal regulation by SnRK2 and COP1 might be a general regulation machinery that controls the stability of dozens of proteins. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-02-20 |
| AnnouncementXML | Submission_2024-02-19_07:00:11.889.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | tian |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; O18 label; ubiquitinylated lysine; sumoylated lysine |
| Instrument | Orbitrap Fusion; Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-02-17 07:02:00 | ID requested | |
| ⏵ 1 | 2024-02-19 07:00:12 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SIZ1 |
| phosphorylation |
| SnRK2 |
| ABA |
| osmotic stress |
Contact List
| Tian Sang | |
|---|---|
| lab head | |
| tian | |
| contact affiliation | Southern University of science and technology |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002048/ |
to receive all new ProteomeXchange dataset release announcements!
