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PXD040231

PXD040231 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and zoites in Plasmodium berghei
DescriptionMalaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
HostingRepositoryPRIDE
AnnounceDate2023-02-21
AnnouncementXMLSubmission_2023-02-21_00:07:21.082.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitteralexandreHainard
SpeciesList scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-17 02:38:40ID requested
12023-02-21 00:07:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cullin1, FBXO-1, gamtocytes, plasmodium berghei,skp1
Contact List
MathieuBrochet
contact affiliationDepartment of Microbiology and Molecular Medicine, CMU, University of Geneva, Geneva, Switzerland
contact emailmathieu.brochet@unige.ch
lab head
alexandreHainard
contact affiliationUniversity of Geneva
contact emailalexandre.hainard@unige.ch
dataset submitter
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Dataset FTP location
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