PXD040188

PXD040188 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoressi (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk
Description	Breast Cancer (BC) is one of the most common cancers and one of the most common causes for cancer- related mortality. Discovery of protein biomarkers associated with cancer, is considered as important for early diagnosis and prediction of the cancer risk. Protein biomarkers could be investigated by large-scale protein investigation or proteomics, using mass spectrometry (MS)- based techniques. Our group applies MS-based proteomics to study the protein pattern in human breast milk from women with BC and controls and investigates the alterations and dysregula-tions of breast milk proteins in comparison pairs of BC versus control. These dysregulated pro-teins might be considered as potential future biomarkers of BC. Identification of potential bi-omarkers in breast milk may benefit young women without BC, but who could collect the milk for future assessment of BC risk. Previously we identified several dysregulated proteins in dif-ferent sets of human breast milk samples from BC patients and controls using gel-based protein separation coupled with MS. Here, we performed two-dimensional polyacrylamide gel electro-phoresis (2D-PAGE) coupled with nano-liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) in a small-scale study on a set of 6 human breast milk samples (3 BC samples vs. 3 controls) and we identified several dysregulated proteins which have potential roles in cancer progression and might be considered as potential BC biomarkers in the future.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:06:14.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danielle Whitham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Xevo Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:29:06	ID requested
1	2023-10-20 11:53:14	announced
⏵ 2	2023-11-14 09:06:23	announced	2023-11-14: Updated project metadata.

Aslebagh R, Whitham D, Channaveerappa D, Lowe J, Pentecost BT, Arcaro KF, Darie CC, Proteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk. Electrophoresis, 44(13-14):1097-1113(2023) [pubmed]

submitter keyword: Breast cancer

Breast milk

Biomarkers

Protein dysregulation

2D-PAGE

Proteomics

Mass spec-trometry

Costel C. Darie
contact affiliation	Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY
contact email	cdarie@clarkson.edu
lab head
Danielle Whitham
contact affiliation	Clarkson University
contact email	whithad@clarkson.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD040188

PRIDE project URI

• PRIDE
  1. PXD040188
     1. Label: PRIDE project
     2. Name: Proteomics analysis of human breast milk by two-dimensional polyacrylamide gel electrophoressi (2D-PAGE) coupled with mass spectrometry to assess breast cancer risk