PXD040186

PXD040186 is an original dataset announced via ProteomeXchange.

Title	RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment
Description	Lassa virus is a negative-strand RNA virus with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming the ribonucleoparticles together with the viral RNA and the L protein, which have to be continuously restructured during viral genome replication and transcription. Z protein is important for ribonucleoparticle recruitment, viral particle assembly and budding, and has also been shown to interact with the L protein. However, the interaction of NP, viral RNA and Z is poorly understood. Here, we characterize the interactions between Lassa virus NP, Z and RNA using structural mass spectrometry. We identify the presence of RNA as the driver for disassembly of ring-like NP trimers into monomers to subsequently form higher order assemblies. We locate the interaction site of Z and NP and demonstrate that while NP binds Z independently of the presence of RNA, this interaction is pH-dependent. These data improve our understanding of ribonucleoparticle assembly and recruitment.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:37:33.862.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lennart Sänger
SpeciesList	scientific name: Lassa mammarenavirus; NCBI TaxID: 11620;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:24:31	ID requested
⏵ 1	2024-10-08 11:37:34	announced

10.1021/JACS.3C07325;

submitter keyword: Native MS,Lassa virus

Charlotte Uetrecht
contact affiliation	Centre for Structural Systems Biology, Leibniz Institute of Virology (LIV), Faculty V: School of Life Sciences, University of Siegen, Deutsches Elektronen-Synchrotron (DESY)
contact email	charlotte.uetrecht@cssb-hamburg.de
lab head
Lennart Sänger
contact affiliation	CSSB
contact email	lennart.saenger@cssb-hamburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD040186

PRIDE project URI

• PRIDE
  1. PXD040186
     1. Label: PRIDE project
     2. Name: RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment