PXD040186 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment |
Description | Lassa virus is a negative-strand RNA virus with only four structural proteins that causes periodic outbreaks in West Africa. The nucleoprotein (NP) encapsidates the viral genome, forming the ribonucleoparticles together with the viral RNA and the L protein, which have to be continuously restructured during viral genome replication and transcription. Z protein is important for ribonucleoparticle recruitment, viral particle assembly and budding, and has also been shown to interact with the L protein. However, the interaction of NP, viral RNA and Z is poorly understood. Here, we characterize the interactions between Lassa virus NP, Z and RNA using structural mass spectrometry. We identify the presence of RNA as the driver for disassembly of ring-like NP trimers into monomers to subsequently form higher order assemblies. We locate the interaction site of Z and NP and demonstrate that while NP binds Z independently of the presence of RNA, this interaction is pH-dependent. These data improve our understanding of ribonucleoparticle assembly and recruitment. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:37:33.862.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lennart Sänger |
SpeciesList | scientific name: Lassa mammarenavirus; NCBI TaxID: 11620; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF; Q-Tof Ultima |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 10:24:31 | ID requested | |
⏵ 1 | 2024-10-08 11:37:34 | announced | |
Publication List
Keyword List
submitter keyword: Native MS,Lassa virus |
Contact List
Charlotte Uetrecht |
contact affiliation | Centre for Structural Systems Biology, Leibniz Institute of Virology (LIV), Faculty V: School of Life Sciences, University of Siegen, Deutsches Elektronen-Synchrotron (DESY) |
contact email | charlotte.uetrecht@cssb-hamburg.de |
lab head | |
Lennart Sänger |
contact affiliation | CSSB |
contact email | lennart.saenger@cssb-hamburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040186
- Label: PRIDE project
- Name: RNA to rule them all: Critical steps in Lassa virus ribonucleoparticle assembly and recruitment