PXD040181

PXD040181 is an original dataset announced via ProteomeXchange.

Title	Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)
Description	To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.
HostingRepository	PRIDE
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-03-20_14:54:01.014.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	NichollasScott
SpeciesList	scientific name: Burkholderia cenocepacia K56-2Valvano; NCBI TaxID: 985076;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:12:07	ID requested
⏵ 1	2023-03-20 14:54:01	announced

Dataset with its publication pending

submitter keyword: glycosylation, CRISPR interference, Burkholderia cenocepacia, glycoproteome,Burkholderia cepacia complex, CRISPRi, glycopeptide enrichment

NichollasScott
contact affiliation	Department of Microbiology and Immunology, The University of Melbourne, Victoria, Australia
contact email	nichollas.scott@unimelb.edu.au
lab head
NichollasScott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD040181

PRIDE project URI

• PRIDE
  1. PXD040181
     1. Label: PRIDE project
     2. Name: Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)