PXD040180

PXD040180 is an original dataset announced via ProteomeXchange.

Title	Expression of spider silk in tobacco improves drought tolerance with minimal effects on its mechanotype
Description	Spider silk, especially dragline silk from Trichonephila clavipes, is an excellent natural material with remarkable mechanical properties. In this study, we generated nucleus- and plastid-encoded expression of a partial sequence of one of the components of dragline silk, major ampullate spidroin-1 (MaSp1), in tobacco (MaSp1-tobacco), MaSp1nuc and MaSp1pla, respectively. The partial sequence of MaSp1 encodes six repetitive glycine-rich and polyalanine tandem domains, and we evaluated the effect of its expression on plant mechanical properties and physiology. While MaSp1 expression showed no effect on leaf mechanical properties, it conferred drought tolerance. MaSp1-tobacco demonstrated an increased growth on PEG-infused growth medium and higher drought recovery in soil compared to the non-transgenic control (WT). Transcriptome analyses of drought-stressed MaSp1-tobacco revealed an upregulation of genes involved in stress response, antioxidant activity, and phenylpropanoid biosynthesis, with a higher effect of drought on the MaSp1pla transcriptome. Genes encoding peroxidase, stress and channel proteins, and cellular metabolism and homeostasis were consistently upregulated in these lines, which also displayed a higher total antioxidant status (TAS) compared to WT, and a higher TAS in MaSp1pla compared to MaSp1nuc. Under normal watering conditions, the ABA content and the TAS in MaSp1-tobacco were significantly higher than in the WT. Our results show that nucleus- and plastid-encoded expression of MaSp1 in tobacco increased the endogenous ABA levels, which led to the formation of plant stress memory that caused overall adjustments in multiple drought tolerance mechanisms and hence, conferred drought tolerance.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:14:13.814.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040180
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Takehiro Suzuki
SpeciesList	scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097;
ModificationList	S-carboxamidoethyl-L-cysteine; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:11:48	ID requested
⏵ 1	2025-05-06 09:14:14	announced

10.6019/PXD040180;

Morey-Yagi SR, Hashida Y, Okamoto M, Odahara M, Suzuki T, Thagun C, Foong CP, Numata K, Expression of spider silk protein in tobacco improves drought tolerance with minimal effects on its mechanotype. Plant J, 121(2):e17213(2025) [pubmed]

10.1111/tpj.17213;

submitter keyword: Spider silk, tensile strength, drought tolerance, glycine-rich, ABA

Naoshi Dohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource
contact email	dohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040180
     1. Label: PRIDE project
     2. Name: Expression of spider silk in tobacco improves drought tolerance with minimal effects on its mechanotype