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PXD040176

PXD040176 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComposition of the mitoribosome of Diplonema papillatum
DescriptionBiosynthesis of mitochondrial genome-encoded proteins is carried out by the mitoribosome, a specialized apparatus that has evolved and diverged dramatically since its bacterial origin. Recent studies across various eukaryotes have demonstrated widespread structural and compositional diversity of mitoribosomes. We used affinity pulldown of four mitoribosomal proteins to carry out a detailed analysis of mitoribosomes in Diplonema papillatum, the type species of diplonemids, a widespread group of single-celled marine flagellates. Using as baits mitoribosomal proteins integrating at distinct sites and phases during subunit maturation also allowed us to sample populations of mitoribosome assembly intermediates.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_13:48:56.273.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarlene Oeffinger
SpeciesList scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationList(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-16 10:06:44ID requested
12024-10-07 13:48:57announced
Publication List
10.1093/nar/gkad422;
Valach M, Benz C, Aguilar LC, Gahura O, Faktorov, á D, Z, í, kov, á A, Oeffinger M, Burger G, Gray MW, Luke, š J, Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway. Nucleic Acids Res, 51(12):6443-6460(2023) [pubmed]
Keyword List
submitter keyword: Diplonema papillatum, flagellate, mitoribosome, assembly intermediate, affinity pulldown,LC-MSMS
Contact List
Marlene Oeffinger
contact affiliationDepartment for Systems Biology, Institut de recherches cliniques de Montréal (IRCM), Montréal, QC, Canada Département de biochimie et médecine moléculaire, Universitéde Montréal, Montréal, QC, Canada Division of Experimental Medicine, McGill University, Montreal, QC, Canada
contact emailMarlene.Oeffinger@ircm.qc.ca
lab head
Marlene Oeffinger
contact affiliationInstitut de recherches cliniques Montreal (IRCM) Universite de Montreal
contact emailmarlene.oeffinger@ircm.qc.ca
dataset submitter
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Dataset FTP location
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