PXD040176

PXD040176 is an original dataset announced via ProteomeXchange.

Title	Composition of the mitoribosome of Diplonema papillatum
Description	Biosynthesis of mitochondrial genome-encoded proteins is carried out by the mitoribosome, a specialized apparatus that has evolved and diverged dramatically since its bacterial origin. Recent studies across various eukaryotes have demonstrated widespread structural and compositional diversity of mitoribosomes. We used affinity pulldown of four mitoribosomal proteins to carry out a detailed analysis of mitoribosomes in Diplonema papillatum, the type species of diplonemids, a widespread group of single-celled marine flagellates. Using as baits mitoribosomal proteins integrating at distinct sites and phases during subunit maturation also allowed us to sample populations of mitoribosome assembly intermediates.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:48:56.273.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlene Oeffinger
SpeciesList	scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:06:44	ID requested
⏵ 1	2024-10-07 13:48:57	announced

10.1093/nar/gkad422;

Valach M, Benz C, Aguilar LC, Gahura O, Faktorov, á D, Z, í, kov, á A, Oeffinger M, Burger G, Gray MW, Luke, š J, Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway. Nucleic Acids Res, 51(12):6443-6460(2023) [pubmed]

submitter keyword: Diplonema papillatum, flagellate, mitoribosome, assembly intermediate, affinity pulldown,LC-MSMS

Marlene Oeffinger
contact affiliation	Department for Systems Biology, Institut de recherches cliniques de Montréal (IRCM), Montréal, QC, Canada Département de biochimie et médecine moléculaire, Universitéde Montréal, Montréal, QC, Canada Division of Experimental Medicine, McGill University, Montreal, QC, Canada
contact email	Marlene.Oeffinger@ircm.qc.ca
lab head
Marlene Oeffinger
contact affiliation	Institut de recherches cliniques Montreal (IRCM) Universite de Montreal
contact email	marlene.oeffinger@ircm.qc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD040176

PRIDE project URI

• PRIDE
  1. PXD040176
     1. Label: PRIDE project
     2. Name: Composition of the mitoribosome of Diplonema papillatum