PXD040174 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Purification of Human β and γ Actin from Budding Yeast |
Description | To facilitate biochemical studies of human cytoplasmic actins, Saccharomyces cerevisiaestrains were developed that express either human β-actin or γ-actin as their sole actin. Actins purified from these strains have biochemical properties similar to mammalian skeletal muscle α-actin, but display differential binding to some key actin regulators. Mass-spectrometry analysis shows that the human actins made from yeast generally retain their N-terminal methionines, which are modified by acetylation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:59:26.407.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD040174 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ebbing de Jong |
SpeciesList | scientific name: Oryctolagus sp. 'Rabbit_OD'; NCBI TaxID: 2665958; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | acetylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 10:06:01 | ID requested | |
1 | 2023-04-24 08:35:45 | announced | |
⏵ 2 | 2023-11-14 06:59:27 | announced | 2023-11-14: Updated project metadata. |
Publication List
10.6019/PXD040174; |
Haarer BK, Pimm ML, de Jong EP, Amberg DC, Henty-Ridilla JL, -actin from budding yeast. J Cell Sci, 136(9):(2023) [pubmed] |
Keyword List
submitter keyword: β-actin,cytoplasmic actin, non-muscle actin, γ-actin |
Contact List
Jessica Henty-Ridilla |
contact affiliation | Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA |
contact email | ridillaj@upstate.edu |
lab head | |
Ebbing de Jong |
contact affiliation | SUNY Upstate Medical University |
contact email | dejonge@upstate.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040174 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040174
- Label: PRIDE project
- Name: Purification of Human β and γ Actin from Budding Yeast