PXD040169

PXD040169 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Affinity-ranking BIN1 interactions and screening natural BIN1 variants that may cause neuromuscular disorders
Description	Deletion of the protein-protein interaction SH3 domain of the membrane remodeling BIN1 protein was found to lead to centronuclear myopathy in patients, yet only few interaction partners of BIN1 SH3 have been identified so far. Here we used the holdup assay to proteome-wide measure steady-state affinity constants of BIN1 SH3 domain for thousands of full-length cellular proteins, as well as for hundreds of putative SH3-binding sites found within the identified BIN1 binders. Besides confirming known partners, such as DNM2, we also identified and affinity-characterized numerous others. We also assessed the impact of a set of rare natural BIN1 SH3 domain variants on affinity interactomes and identified a set of potentially harmful ones that exhibited perturbed affinity profiles, whose impact were confirmed by cellular assay, tentatively connecting them to neuromuscular disorders.
HostingRepository	PRIDE
AnnounceDate	2024-07-11
AnnouncementXML	Submission_2024-07-11_09:48:53.714.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Negroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 10:00:34	ID requested
⏵ 1	2024-07-11 09:48:54	announced

Publication List

10.7554/ELIFE.95397;

10.7554/ELIFE.95397;

Keyword List

submitter keyword: BIN1 interactions,native holdup

submitter keyword: BIN1 interactions,native holdup

Contact List

Gergo Gogl
contact affiliation	Équipe Labellisée Ligue 2015, Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258/CNRS UMR 7104/Université de Strasbourg, 1 rue Laurent Fries, BP 10142, F-67404 Illkirch, France
contact email	goglg@igbmc.fr
lab head
Luc Negroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040169
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040169

PRIDE project URI

Repository Record List

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