PXD040169 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Affinity-ranking BIN1 interactions and screening natural BIN1 variants that may cause neuromuscular disorders |
Description | Deletion of the protein-protein interaction SH3 domain of the membrane remodeling BIN1 protein was found to lead to centronuclear myopathy in patients, yet only few interaction partners of BIN1 SH3 have been identified so far. Here we used the holdup assay to proteome-wide measure steady-state affinity constants of BIN1 SH3 domain for thousands of full-length cellular proteins, as well as for hundreds of putative SH3-binding sites found within the identified BIN1 binders. Besides confirming known partners, such as DNM2, we also identified and affinity-characterized numerous others. We also assessed the impact of a set of rare natural BIN1 SH3 domain variants on affinity interactomes and identified a set of potentially harmful ones that exhibited perturbed affinity profiles, whose impact were confirmed by cellular assay, tentatively connecting them to neuromuscular disorders. |
HostingRepository | PRIDE |
AnnounceDate | 2024-07-11 |
AnnouncementXML | Submission_2024-07-11_09:48:53.714.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luc Negroni |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-16 10:00:34 | ID requested | |
⏵ 1 | 2024-07-11 09:48:54 | announced | |
Publication List
Keyword List
submitter keyword: BIN1 interactions,native holdup |
Contact List
Gergo Gogl |
contact affiliation | Équipe Labellisée Ligue 2015, Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258/CNRS UMR 7104/Université de Strasbourg, 1 rue Laurent Fries, BP 10142, F-67404 Illkirch, France |
contact email | goglg@igbmc.fr |
lab head | |
Luc Negroni |
contact affiliation | CNRS |
contact email | luc.negroni@igbmc.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040169 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040169
- Label: PRIDE project
- Name: Affinity-ranking BIN1 interactions and screening natural BIN1 variants that may cause neuromuscular disorders