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PXD040169

PXD040169 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAffinity-ranking BIN1 interactions and screening natural BIN1 variants that may cause neuromuscular disorders
DescriptionDeletion of the protein-protein interaction SH3 domain of the membrane remodeling BIN1 protein was found to lead to centronuclear myopathy in patients, yet only few interaction partners of BIN1 SH3 have been identified so far. Here we used the holdup assay to proteome-wide measure steady-state affinity constants of BIN1 SH3 domain for thousands of full-length cellular proteins, as well as for hundreds of putative SH3-binding sites found within the identified BIN1 binders. Besides confirming known partners, such as DNM2, we also identified and affinity-characterized numerous others. We also assessed the impact of a set of rare natural BIN1 SH3 domain variants on affinity interactomes and identified a set of potentially harmful ones that exhibited perturbed affinity profiles, whose impact were confirmed by cellular assay, tentatively connecting them to neuromuscular disorders.
HostingRepositoryPRIDE
AnnounceDate2024-07-11
AnnouncementXMLSubmission_2024-07-11_09:48:53.714.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuc Negroni
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-02-16 10:00:34ID requested
12024-07-11 09:48:54announced
Publication List
10.7554/ELIFE.95397;
Keyword List
submitter keyword: BIN1 interactions,native holdup
Contact List
Gergo Gogl
contact affiliationÉquipe Labellisée Ligue 2015, Département de Biologie Structurale Intégrative, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258/CNRS UMR 7104/Université de Strasbourg, 1 rue Laurent Fries, BP 10142, F-67404 Illkirch, France
contact emailgoglg@igbmc.fr
lab head
Luc Negroni
contact affiliationCNRS
contact emailluc.negroni@igbmc.fr
dataset submitter
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