PXD040163

PXD040163 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomics of microbiota-derived indole metabolites in mammalian cells
Description	The microbiota generates diverse metabolites to modulate host physiology and disease, but their protein targets and mechanisms of action have not been fully elucidated. To address this challenge, we focused on microbiota-derived indole metabolites and develop photoaffinity chemical probes for proteomic studies. We identified many potential indoleinteracting proteins, including metabolic enzymes, transporters, immune sensors, and Gprotein coupled receptors. Notably, we discovered aromatic monoamines can activate - arrestin recruitment by the orphan receptor GPRC5A. Metabolomic and functional profiling of microbiota species revealed specific aromatic amino acid decarboxylaseexpressing bacteria producing monoamine agonists for GPRC5A. Structure-activity relationship studies of synthetic aromatic monoamines identified 7-fluorotryptamine as a more potent activator of -arrestin recruitment by GPRC5A. Our results highlight the utility of chemoproteomics to identify novel microbiota metabolite-interacting proteins and discover potential microbiota-derived small molecule agonists for orphan receptors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:55:55.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaohui Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 09:51:18	ID requested
1	2023-05-31 10:01:48	announced
⏵ 2	2023-11-14 08:55:56	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Bacterial metabolites, Mammalian cells, Chemical proteomics, photoaffinity probes

Howard C. Hang
contact affiliation	Department of Immunology and Microbiology, Scripps Research, USA
contact email	hhang@scripps.edu
lab head
Xiaohui Zhao
contact affiliation	Scripps Research Institute
contact email	xzhao@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD040163

PRIDE project URI

• PRIDE
  1. PXD040163
     1. Label: PRIDE project
     2. Name: Chemoproteomics of microbiota-derived indole metabolites in mammalian cells