PXD040161

PXD040161 is an original dataset announced via ProteomeXchange.

Title	Rescue of Escherichia coli auxotrophy by de novo small proteins
Description	Increasing numbers of small proteins with diverse physiological roles are being identified and characterized in both prokaryotic and eukaryotic systems, but the origins and evolution of these proteins remain unclear. Recent genomic sequence analyses in several organisms suggest that new functions encoded by small open reading frames (sORFs) may emerge de novo from noncoding sequences. However, experimental data demonstrating if and how randomly generated sORFs can confer beneficial effects to cells are limited. Here we show that by up-regulating hisB expression, de novo small proteins (≤ 50 amino acids in length) selected from random sequence libraries can rescue Escherichia coli cells that lack the conditionally essential SerB enzyme. The recovered small proteins are hydrophobic and confer their rescue effect by binding to the 5’ end regulatory region of the his operon mRNA, suggesting that protein binding promotes structural rearrangements of the RNA that allow increased hisB expression. This study adds RNA regulatory elements as another interacting partner for de novo proteins isolated from random sequence libraries, and provides further experimental evidence that small proteins with selective benefits can originate from the expression of nonfunctional sequences.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:21.951.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mårten Larsson
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 09:50:36	ID requested
⏵ 1	2023-11-14 09:00:22	announced

Dataset with its publication pending

submitter keyword: Escherichia coli, RNA-binding, de novo gene evolution, gene regulation, his operon, auxotroph,small proteins

Dan I. Andersson
contact affiliation	Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
contact email	Dan.Andersson@imbim.uu.se
lab head
Mårten Larsson
contact affiliation	Uppsala University
contact email	Marten.Larsson@imbim.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD040161
     1. Label: PRIDE project
     2. Name: Rescue of Escherichia coli auxotrophy by de novo small proteins