PXD040147

PXD040147 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins differentially associated with SARS CoV-2 subgenomic RNAs
Description	We characterize the specific protein interactomes of 3 SARS sgRNAs (S, N, and ORF8) as well as the gRNA at two time points, 8- and 24- hours. These two timepoints were selected to capture early viral infection when RNA synthesis is just beginning, and viral replication is being established, as well as a later stage where protein synthesis and virion packaging is occurring. We identified over 500 proteins associated with one or more of the viral RNAs. These include both host and viral proteins that have been previously shown to bind to SARS-CoV-2 RNAs and novel (not previously identified) protein interactors.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:24.419.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Isabella Whitworth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-16 07:55:45	ID requested
1	2023-07-30 16:19:39	announced
⏵ 2	2023-11-14 09:04:25	announced	2023-11-14: Updated project metadata.

Whitworth IT, Knoener RA, Puray-Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM, Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs. bioRxiv, ():(2023) [pubmed]

submitter keyword: viral,proteomics, S RNA, mass spectrometry, N RNA, RNA-protein interactions, RNA metabolism, gRNA, interactome, SARS CoV-2, hybridization, ORF8 RNA, RNA binding proteins

Identification of proteins differentially associated with SARS CoV-2 subgenomic RNAs
contact affiliation	Department of Chemistry University of Wisconsin-Madison
contact email	smith@chem.wisc.edu
lab head
Isabella Whitworth
contact affiliation	University of Wisconsin-Madison
contact email	iwhitworth@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD040147

PRIDE project URI

• PRIDE
  1. PXD040147
     1. Label: PRIDE project
     2. Name: Identification of proteins differentially associated with SARS CoV-2 subgenomic RNAs