PXD040125 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global interactome mapping reveals pro-cancerous interactions of NF-κB in breast cancer |
Description | NF-κB has an essential role in innate immune response and inflammation and is involved in cancer development and progression. We apply the SEC-PCP-SILAC method incorporating metabolic labeling, size exclusion chromatography and protein correlation profiling to construct a complex network of interactome rearrangement in response to NF-κB modulation in breast cancer cells. Our interaction network represents a complex insight into the dynamics of MCF-7 protein interactome associated with NF-κB pathway. Our dataset could serve as a basis for future studies characterizing role of NF-κB in breast cancer cellular pathways. This PRIDE project includes results from SILAC labeled and label-free replicates from the SEC-PCP-SILAC analysis of protein complexes in MCF-7 cells with inhibited and uninhibited NF-κB pathway, results from the immunoprecipitation experiment aimed at interaction partners of NF-κB factor RELA, analysis of total proteome after NF-κB inhibition, and results from SEC fractionation of untreated and unlabeled MCF-7 cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-29 |
AnnouncementXML | Submission_2024-02-29_03:26:50.435.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavel Bouchal |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-15 08:23:18 | ID requested | |
⏵ 1 | 2024-02-29 03:26:50 | announced | |
Publication List
Keyword List
ProteomeXchange project tag: EPIC-XS |
submitter keyword: NF-κB, RELA, Breast cancer, Protein correlation profiling, Protein complexes, Interaction, Proteomics |
Contact List
Pavel Bouchal |
contact affiliation | Masaryk University, Faculty of Science |
contact email | bouchal@chemi.muni.cz |
lab head | |
Pavel Bouchal |
contact affiliation | Masaryk University, Faculty of Science |
contact email | bouchal@chemi.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD040125 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040125
- Label: PRIDE project
- Name: Global interactome mapping reveals pro-cancerous interactions of NF-κB in breast cancer