PXD040117

PXD040117 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chlorophyll biosynthesis under the control of arginine metabolism
Description	In natural environments, photosynthetic organisms adjust their metabolism to cope with the fluctuating availability of combined nitrogen-sources, a growth limiting factor. For the acclimation, the dynamic degradation/synthesis of tetrapyrrolic pigments as well as of the amino acid arginine is pivotal; however, there was no evidence that these processes could be functionally coupled. Using co-immunopurification and spectral shift assays we found that in the cyanobacterium Synechocystis sp. PCC 6803 the arginine-related ArgD and CphB enzymes form protein complexes with Gun4, an essential factor for chlorophyll biosynthesis. Gun4 binds ArgD with high affinity, and the ArgD-Gun4 complex strongly accumulates in cells supplemented with ornithine, a key intermediate of the arginine pathway. Elevated ornithine levels restricted de novo synthesis of tetrapyrrolic pigments, which arrested the recovery from nitrogen deficiency. Our data reveals a direct cross-talk between tetrapyrrole biosynthesis and arginine metabolism that clarifies the importance of balancing photosynthetic pigment synthesis with nitrogen homeostasis.
HostingRepository	MassIVE
AnnounceDate	2023-09-03
AnnouncementXML	Submission_2023-09-04_00:56:21.848.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Konik
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	Oxidation; Carbamidomethyl; Deamidated
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-14 07:46:20	ID requested
⏵ 1	2023-09-04 00:56:22	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: tetrapyrrole biosynthesis, Synechocystis, chlorophyll, bilins, Genome-Uncoupled-4, arginine metabolism, nitrogen homeostasis

submitter keyword: tetrapyrrole biosynthesis, Synechocystis, chlorophyll, bilins, Genome-Uncoupled-4, arginine metabolism, nitrogen homeostasis

Contact List

Roman Sobotka
contact affiliation	1Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Trebon; Faculty of Science, University of South Bohemia, 37005, Ceske Budejovice
contact email	sobotka@alga.cz
lab head
Peter Konik
contact affiliation	University of South Bohemia, Ceske Budejovice
contact email	konik@prf.jcu.cz
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091280/