PXD040110

PXD040110 is an original dataset announced via ProteomeXchange.

Title	Multi-omics AnalysisIntegrated Proteomic and Metabolomic Analysis Reveals Underlying Host Responses in Pediatric Respiratory Syncytial Virus Pneumonia
Description	Despite efforts made to reveal the underlying cause for differences in host responses and severity to Rrespiratory syncytial virus (RSV) infectionis an important pathogen causing pneumonia in children, few studies have used multi-omics data to investigate the pathogenies of RSV pneumonia. Here, metabolomics was first used to identify potential biomarkers for RSV diagnosis. In the training cohort, serum from 36 healthy controls (HCs), 45 RSV pneumonia children with respiratory syncytial virus pneumonia (RSVs), and 32 other infectious disease controls (IDCs) were recruited. After multivariate analyses, 283 metabolites were differently expressed in RSV compared to the HC and IDC groups. Among them, 6 metabolites were shown to have potential diagnostic values. Using an independent cohort of 49 subjects, 2 potential biomarkers (neuromedin N and histidylproline diketopiperazine) were validated. Next, a combination of multi-omics analysisproteomics and metabolomics were applied to analyze the pathogenies of protein-metabolite crosstalk in RSV pneumonia. Accumulation of collagen in the serum of RSVs indicated that RSV infection could lead to increased levels of soluble collagen, which may help to stimulate airway remodeling of the lungs. Additionally, activation of the complement system and the inflammatory responseimbalance in lipid metabolism were also observed in RSV patients. Finally, an imbalance in lipid metabolism, including glycerolipids, glycerophospholipids, sphingolipids, and prenol lipids, accompanied with apolipoprotein abnormalities were suggested to be involved in the pathogenesis of RSV pneumonia. The multi-omics analysis presented in our study here revealed the signature protein and metabolite changes in serum caused by RSV infection. This data provides essential diagnostic and therapeutic information in the fight against RSV infection in children.
HostingRepository	iProX
AnnounceDate	2023-02-14
AnnouncementXML	Submission_2023-03-28_20:29:35.082.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jieqiong Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-13 18:08:34	ID requested
1	2023-02-13 18:08:51	announced
⏵ 2	2023-03-28 20:29:35	announced	2023-03-29: Update publication information.

Huang X, Li F, Wang Y, Jia X, Jia N, Xiao F, Sun C, Fu J, Chen M, Cui X, Qu D, Wai Luu LD, Tai J, Li J, Multi-omics analysis reveals underlying host responses in pediatric respiratory syncytial virus pneumonia. iScience, 26(4):106329(2023) [pubmed]

submitter keyword: Respiratory syncytial virus pneumonia，Proteomics，Metabolomics，Diagnosis，Protein-Metabolites Crosstalk.

Jieqiong Li
contact affiliation	Beijing Chaoyang Hospital
contact email	jieqiongli2010@163.com
lab head
Jieqiong Li
contact affiliation	Beijing Chaoyang Hospital
contact email	jieqiongli2010@163.com
dataset submitter

iProX dataset URI