PXD040105

PXD040105 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of lungs in early experimental model of Chronic obstructive pulmonary disease (COPD)
Description	Rationale: Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory disease lacking disease modifying treatment. The role of CXCL12/CXCR4 axis has been demonstrated in acute exacerbation of COPD. The interest of study early COPD has been recently pointed out. Objectives: To study the role of the CXCL12/CXCR4 axis in both human and mouse model of early COPD. Methods: Blood and lung tissue were obtained from both COPD patients and mice exposed to cigarette smoke (CS) for 10 weeks and intranasal instillations of polyinosinic–polycytidylic acid (poly(I:C)) to mimic exacerbations for 5 weeks. Measurements and Main Results: Exposed mice presented mild airway obstruction, peri-bronchial fibrosis and right heart remodeling. The level of CXCR4 expressing cells was increased in the blood of exposed mice, as well as in the blood of patients with mild COPD. Lung CXCL12 expression was higher both in exposed mice and COPD patients. The densities of fibrocytes expressing CXCR4 were increased in the blood and in the bronchial submucosa of exposed mice. Conditional inactivation of CXCR4 at adult stage as well as pharmacological inhibition of CXCR4 with plerixafor injections improved lung function and inflammation and protected against CS and poly-(I:C)-induced airway and cardiac remodeling. CXCR4-/- and plerixafor-treated mice also had reduced levels of CXCR4-expressing circulating cells and a lower density of peri-bronchial fibrocytes. Conclusions: We demonstrated that targeting CXCR4 has beneficial effects in an animal model of early COPD, and provide a framework to translate preclinical findings to clinical settings within a drug repurposing approach.
HostingRepository	PRIDE
AnnounceDate	2025-05-12
AnnouncementXML	Submission_2025-05-12_00:28:50.495.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-13 13:32:59	ID requested
⏵ 1	2025-05-12 00:28:51	announced

10.1165/rcmb.2024-0303oc;

Dupin I, Henrot P, Maurat E, Abohalaka R, Chaigne S, El Hamrani D, Eyraud E, Prevel R, Esteves P, Campagnac M, Dubreuil M, Cardouat G, Bouchet C, Ousova O, Dupuy JW, Trian T, Thumerel M, Begueret H, Girodet PO, Marthan R, Zysman M, Freund-Michel V, Berger P, CXCR4 Blockade Alleviates Pulmonary and Cardiac Outcomes in Early COPD. Am J Respir Cell Mol Biol, ():(2025) [pubmed]

submitter keyword: Lungs, quantitative proteome, CXCR4, cigarette smoke, plerixafor, chronic obstructive disease

Isabelle Dupin
contact affiliation	Centre de recherche Cardio-Thoracique de Bordeaux, INSERM U1045, PTIB, Hôpital Xavier Arnozan, Avenue du Haut Lévêque, 33604 Pessac Cedex
contact email	isabelle.dupin@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD040105

PRIDE project URI

• PRIDE
  1. PXD040105
     1. Label: PRIDE project
     2. Name: Proteomic analysis of lungs in early experimental model of Chronic obstructive pulmonary disease (COPD)