PXD040103 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Optimising complexome profiling for systematic exploration of DNA-/RNA-protein complexes in human mitochondria |
Description | Here we aimed to improve native gel electrophoresis-based complexome profiling (CP) method for examination of mitochondrial (mt)DNA-/RNA-protein complexes. We compared the performance of Blue Native PAGE (BNE) and high resolution Clear Native PAGE (hrCNE). We found that hrCNE much better preserves the integrity of mtDNA-/RNA-protein complexes.To further improve the detection of mtDNA-binding proteins, we treated solubilized mitochondrial samples with DNase I prior to separation on hrCNE or BNE. To characterize the detected by hrCNE mtRNA-protein complexes, we performed CP with samples treated with RNase A. To validate the utility of the method, we performed CP using mitochondria isolated from cells treated with ethidium bromide as well as from cells recovering after this treatment. Our study not only helps to validate and unveil proteins involved in mitochondrial DNA- and RNA-related processes, but also offers a convenient and systematic way for analysing these interactions that can be used equally well for the investigation of the nucleus and other DNA-/RNA-containing cell compartments. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:00:09.336.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alisa Potter |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-02-13 10:30:57 | ID requested | |
1 | 2023-08-11 14:32:34 | announced | |
2 | 2023-11-14 06:43:27 | announced | 2023-11-14: Updated project metadata. |
⏵ 3 | 2024-10-22 06:00:09 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: mtDNA,Mitochondria, mass spectrometry, DNA-protein complex, native gel electrophoresis, RNA-protein complex, complexome profiling |
Contact List
Johannes N. Spelbrink |
contact affiliation | Radboud Center for Mitochondrial Medicine, Department of Paediatrics, Radboudumc, Nijmegen, The Netherlands |
contact email | Hans.Spelbrink@radboudumc.nl |
lab head | |
Alisa Potter |
contact affiliation | Radboudumc, Department of Paediatrics |
contact email | potteralisa@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD040103 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD040103
- Label: PRIDE project
- Name: Optimising complexome profiling for systematic exploration of DNA-/RNA-protein complexes in human mitochondria