PXD040094

PXD040094 is an original dataset announced via ProteomeXchange.

Title	Proteomic response on ferroptosis induction via GPX4 KO in mouse embryonic fibroblasts
Description	In the present study, we report proteome data of mouse embryonic fibroblasts (MEF cells) and immortalized mouse embryonic fibroblasts which are 4-OH-TAM-inducible Gpx4−/− (Pfa1 cells). Ferroptosis in Pfa1 cells was induced with Tamoxifen (Tam) addition and three timepoints were collected: 0h, 24h and 48h. Wild-type MEF cells were also measured as an alternative control without Tam addition. Raw data for HEK293 cells are provided as an independent quality control. MEF and Pfa1 cells (both kind gift from Marcus Conrad, Munich) were cultured in RPMI-1640 media (2 g/L glucose, 10% FBS, 2 mM Gibco GlutaMAX Supplement, 1% pen/strep) at 37C with 5% CO2 in a humidified incubator. Cells (300k) were seeded on Corning 100 mm tissue-culture treated culture dishes and incubated overnight. On the next day Pfa1 cells were treated with or without 1 µM Tam.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:00:06.404.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040094
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexey Nesterenko
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-13 03:35:57	ID requested
1	2023-04-12 07:48:54	announced
2	2023-05-03 02:50:11	announced	2023-05-03: Updated project metadata.
⏵ 3	2023-11-14 09:00:07	announced	2023-11-14: Updated project metadata.

10.6019/PXD040094;

submitter keyword: proteomics, mouse embryonic fibroblasts,Ferroptosis, glutathione peroxidase 4, Pfa1, cell death

Vsevolod V. Belousov
contact affiliation	Federal Center of Brain Research and Neurotechnologies, Federal Medico-Biological Agency, 117513 Moscow, Russia
contact email	vsevolod.belousov@gmail.com
lab head
Alexey Nesterenko
contact affiliation	Federal Center of Brain Research and Neurotechnologies
contact email	comcon1@protonmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD040094

PRIDE project URI

• PRIDE
  1. PXD040094
     1. Label: PRIDE project
     2. Name: Proteomic response on ferroptosis induction via GPX4 KO in mouse embryonic fibroblasts