PXD040073

PXD040073 is an original dataset announced via ProteomeXchange.

Title	TurboID-based proximity labeling identifies reticulon-like protein B2 as a pro-viral factor coopted for the biogenesis of viral replication organelles in plants
Description	Although intensive studies have been performed to identify and characterize host factors involved in viral replication complex formation, the high dynamism of this process and the insoluble properties of the complex make it difficult to be analyzed comprehensively using traditional affinity purification methods. TurboID-based proximity labeling emerges as a robust tool for probing molecular interactions in planta, yet there is no report on the application of TurboID on the study of plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum-replicating virus, as a model, and systematically investigated the composition of BBSV virus replication complex (VRC) by fusing the TurboID enzyme to viral replicase protein p23. Among the 185 identified p23-proximal proteins, reticulon family proteins showed high reproducibility in the mass spectrometry datasets. We focused on the reticulon-like protein B2 (RTNLB2) and demonstrated its pro-viral functions in BBSV replication. Mechanistic analysis revealed that RTNLB2 binds to p23, induces the ER membrane curvature and constricts ER tubules to facilitate the assembly of BBSV VRC. Our comprehensive proximal interactome analysis of BBSV VRC provides a resource for understanding of plant viral replication and offers new insights into the formation of membrane scaffolds for viral RNA synthesis.
HostingRepository	iProX
AnnounceDate	2023-02-12
AnnouncementXML	Submission_2023-05-23_19:17:23.716.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyan Wen
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	TMT6plex reporter fragment
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-12 19:23:34	ID requested
⏵ 1	2023-05-23 19:17:24	announced

Zhang Q, Wen Z, Zhang X, She J, Wang X, Gao Z, Wang R, Zhao X, Su Z, Li Z, Li D, Wang X, Zhang Y, RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants. Plant Cell, 35(8):3127-3151(2023) [pubmed]

submitter keyword: TurboID-based proximity labeling (PL), Virus replication complex (VRC), ER, Beet black scorch virus (BBSV), Interactome, Reticulon-like protein B2, Membrane curvature, Assembly

Yongliang Zhang
contact affiliation	China Agricultural University
contact email	cauzhangyl@cau.edu.cn
lab head
Zhiyan Wen
contact affiliation	China Agricultural University
contact email	zhiyanwen@cau.edu.cn
dataset submitter

iProX dataset URI