PXD040008

PXD040008 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Overexpression of the citrate transporters SLC13A5 and SLC25A1 in the mouse elicit different metabolic responses and phenotypes
Description	Cytosolic citrate is imported from the mitochondria by SLC25A1, and from the extracellular milieu by SLC13A5. In the cytosol, citrate is used by ACLY to generate acetyl-CoA, which can then be exported to the endoplasmic reticulum (ER) by SLC33A1. Here, we report the generation of mice with systemic overexpression (sTg) of SLC25A1 or SLC13A5. Both animals displayed increased cytosolic levels of citrate and acetyl-CoA; however, SLC13A5 sTg mice developed a progeria-like phenotype with premature death, while SLC25A1 sTg mice did not. Analysis of the metabolic profile revealed widespread differences. Furthermore, SLC13A5 sTg mice displayed increased engagement of the ER acetylation machinery through SLC33A1, while SLC25A1 sTg mice did not. In conclusion, our findings point to different biological responses to SLC13A5- or SLC25A1-mediated import of citrate and suggest that the directionality of the citrate/acetyl-CoA pathway can transduce different signals. Results uploaded here in MassIVE are acetyl-proteomics studies of the livers of SLC13A5 and SLC25A1 mice as compared to their WT litter-mates.
HostingRepository	MassIVE
AnnounceDate	2023-09-12
AnnouncementXML	Submission_2023-09-12_12:12:58.208.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexis Lawton
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Acetyl; Acetyl:2H(3)
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 11:09:15	ID requested
⏵ 1	2023-09-12 12:12:58	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Acetylation, Proteomics, Subcellular

submitter keyword: Acetylation, Proteomics, Subcellular

Contact List

John Denu
contact affiliation	University of Wisconsin - Madison
contact email	john.denu@wisc.edu
lab head
Alexis Lawton
contact affiliation	University of Wisconsin - Madison
contact email	alawton2@wisc.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091235/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000091235/