PXD039998

PXD039998 is an original dataset announced via ProteomeXchange.

Title	EP300 promotes ferroptosis via HSPA5 acetylation in pancreatic cancer
Description	Ferroptosis is a form of regulated cell death characterized by oxidative injury-induced lipid peroxidation. However, the detailed protein post-translational modification regulatory mechanism of ferroptosis remains largely unknown. Here, we report that E1A binding protein P300 (EP300) acetyltransferase promotes ferroptosis in human pancreatic ductal adenocarcinoma (PDAC) cells via the acetylation of heat shock protein family A (Hsp70) member 5 (HSPA5, also known as GRP78 or BIP) on the site of K353. Acetylated HSPA5 loses its ability to inhibit lipid peroxidation and subsequent ferroptotic cell death. Genetic or pharmacological inhibition of EP300-mediated HSPA5 acetylation on K353 increases PDAC cell resistance to ferroptosis. Moreover, histone deacetylase 6 (HDAC6) limits HSPA5 acetylation and subsequent ferroptosis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:07:51.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuan Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 01:55:46	ID requested
1	2023-10-24 11:49:35	announced
⏵ 2	2023-11-14 09:07:57	announced	2023-11-14: Updated project metadata.

Wang Y, Liu Y, Wang C, Kang R, Tang D, Liu J, EP300 promotes ferroptosis via HSPA5 acetylation in pancreatic cancer. Sci Rep, 13(1):15004(2023) [pubmed]

submitter keyword: Human, Cell, IP-MS, PDAC

Jiao Liu
contact affiliation	The Third Affiliated Hospital of Guangzhou Medical University
contact email	641504008@qq.com
lab head
Yuan Wang
contact affiliation	The Third Affiliated Hospital of Guangzhou Medical University
contact email	1067163037@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039998
     1. Label: PRIDE project
     2. Name: EP300 promotes ferroptosis via HSPA5 acetylation in pancreatic cancer