PXD039995

PXD039995 is an original dataset announced via ProteomeXchange.

Title	A combinatorial approach to uncover an additional Integrator subunit
Description	RNA Polymerase II (RNAPII) controls expression of all protein coding genes and most noncoding loci in higher eukaryotes. Calibrating RNAPII activity requires an assortment of polymerase-associated factors that are recruited at sites of active transcription. The Integrator complex is one of the most elusive transcriptional regulators in metazoans, deemed to be recruited after initiation to help establish and modulate paused RNAPII. Integrator is known to be composed of 14 subunits that assemble and operate in a modular fashion. We employed proteomics and machine-learning structure prediction (AlphaFold2) to identify an additional Integrator subunit, INTS15. We report that INTS15 assembles primarily with the INTS13/INTS14/INTS10 module and interfaces with the Int-PP2A module. Functional genomics analysis further reveals a role for INTS15 in modulating RNAPII pausing at a subset of genes. Our study shows that omics approaches combined with AlphaFold2-based predictions provide additional insights into the molecular architecture of large and dynamic multiprotein complexes.
HostingRepository	PRIDE
AnnounceDate	2023-02-10
AnnouncementXML	Submission_2023-02-10_08:20:25.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SarahOffley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 01:51:54	ID requested
⏵ 1	2023-02-10 08:20:26	announced

Dataset with its publication pending

submitter keyword: Human, IP-MS, LC-MS/MS

AlessandroGardini
contact affiliation	The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19103, USA
contact email	agardini@wistar.org
lab head
SarahOffley
contact affiliation	Wistar Institute
contact email	soffley@wistar.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD039995

PRIDE project URI

• PRIDE
  1. PXD039995
     1. Label: PRIDE project
     2. Name: A combinatorial approach to uncover an additional Integrator subunit