PXD039992

PXD039992 is an original dataset announced via ProteomeXchange.

Title	OMICS Analyses Unraveling Related Gene and Protein Driven Molecular Mechanisms Underlying PACAP 38 Induced Neurite Outgrowth in PC12 Cells
Description	Recently, our group has shown that the pituitary adenylate cyclase-activating polypeptide (PACAP)-induced neurite projection elongation in rat adrenal-derived pheochromocytoma cell line (PC12) is mediated via PAC1 receptor-mediated dephosphorylation of CRMP2 majorly through PI3K/AKT and MEK/ERK pathways. However, the mechanism of neuronal outgrowth by PACAP, including CRMP2 dephosphorylation, remains unclear. In our previous report, we found that GSK-3β, CDK5, and Rho/ROCK dephosphorylated CRMP2 within 3 hours after the addition of PACAP, but the early dephosphorylation of CRMP2 by PACAP remains unclear. Thus, we considered it important to identify early factors in PACAP-induced neurite projection elongation and performed omics-based transcriptomic (whole genome DNA microarray) and proteomic (TMT-labeling in conjunction with liquid chromatography-tandem mass spectrometry) analyses of gene and protein expression profiles from 5-120 minutes after PACAP addition. Results surprisingly revealed a number of key regulators involved in neurite outgrowth, including known ones. We also identified factors that may be involved in CRMP2 dephosphorylation. Cross-referencing previous research, we tried to map these molecular components onto potential pathways. The results of this comprehensive analysis may provide important new information on the molecular mechanisms of neuronal differentiation induced by PACAP.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:43:59.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Randeep Rakwal
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 01:44:19	ID requested
1	2023-03-11 11:40:40	announced
⏵ 2	2023-11-14 08:44:07	announced	2023-11-14: Updated project metadata.

Shibato J, Takenoya F, Yamashita M, Gupta R, Min CW, Kim ST, Kimura A, Takasaki I, Hori M, Shioda S, Rakwal R, OMICS Analyses Unraveling Related Gene and Protein-Driven Molecular Mechanisms Underlying PACAP 38-Induced Neurite Outgrowth in PC12 Cells. Int J Mol Sci, 24(4):(2023) [pubmed]

submitter keyword: Rat, TMT, PC12, Proteomics, Neurite

Randeep Rakwal
contact affiliation	Institute of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8574, Ibaraki, Japan
contact email	plantproteomics@gmail.com
lab head
Randeep Rakwal
contact affiliation	University of Tsukuba, Tsukuba
contact email	plantproteomics@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039992
     1. Label: PRIDE project
     2. Name: OMICS Analyses Unraveling Related Gene and Protein Driven Molecular Mechanisms Underlying PACAP 38 Induced Neurite Outgrowth in PC12 Cells