PXD039991

PXD039991 is an original dataset announced via ProteomeXchange.

Title	The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation.
Description	MOM1 is an Arabidopsis factor previously shown to mediate transcriptional silencing independent of major DNA methylation changes. Here we found that MOM1 localizes with sites of RNA-directed DNA methylation (RdDM). Tethering MOM1 with artificial zinc finger to unmethylated FWA promoter led to establishment of DNA methylation and FWA silencing. This process was blocked by mutations in components of the Pol V arm of the RdDM machinery, as well as by mutation of MORC6. We found that at some endogenous RdDM sites, MOM1 is required to maintain DNA methylation and a closed chromatin state. In addition, efficient silencing of newly introduced FWA transgenes was impaired by mutation of MOM1 or mutation of genes encoding the MOM1 interacting PIAL1/2 proteins. In addition to RdDM sites, we identified a group of MOM1 peaks at active chromatin near genes that colocalized with MORC6. These findings demonstrate a multifaceted role of MOM1 in genome regulation.
HostingRepository	PRIDE
AnnounceDate	2023-06-10
AnnouncementXML	Submission_2023-06-09_18:40:26.712.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YasamanJami-Alahmadi
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 01:44:02	ID requested
⏵ 1	2023-06-09 18:40:27	announced

Dataset with its publication pending

submitter keyword: MOM1, arabidopsis,LCMS

steveJacobsen
contact affiliation	ucla
contact email	jacobsen@ucla.edu
lab head
YasamanJami-Alahmadi
contact affiliation	UCLA
contact email	yjami@ucla.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD039991
     1. Label: PRIDE project
     2. Name: The MOM1 complex recruits the RdDM machinery via MORC6 to establish de novo DNA methylation.