PXD039980

PXD039980 is an original dataset announced via ProteomeXchange.

Title	Saddleback caterpillar venom proteomics
Description	Limacodidae is a family of lepidopteran insects comprising more than 1,500 species, over half of which produce pain-inducing defensive venom components in the larval stage, but little is known about their venom. Recently, we characterised proteinaceous toxins from the Australian limacodid caterpillar Doratifera vulnerans, but it is unknown if the venom of this species is typical of other Limacodidae. Here, we use single animal transcriptomics and venom proteomics to investigate the venom of an iconic limacodid, the North American saddleback caterpillar Acharia stimulea. We identified 65 confirmed venom polypeptides, grouped into 31 different families. Neurohormones, knottins, and homologues of the immune signaller Diedel make up the majority of A. stimulea venom, indicating strong similarities to D. vulnerans venom, despite their geographic separation. One notable difference is the presence of RF-amide peptide toxins in A. stimulea venom. Synthetic versions of these RF-amide toxins displayed potent activation of the human neuropeptide FF1 receptor, insecticidal activity when injected into Drosophila melanogaster, and moderate ability to inhibit the larval development of the parasitic nematode Haemonchus contortus. This study provides insights into venom evolution and toxin activity in Limacodidae, and will allow the synthetic production of A. stimulea peptide toxins towards functional and structural characterisation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:30.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohaddeseh Goudarzi
SpeciesList	scientific name: Acharia stimulea; NCBI TaxID: 691692;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-09 00:56:24	ID requested
1	2023-06-01 08:02:49	announced
⏵ 2	2023-11-14 08:48:35	announced	2023-11-14: Updated project metadata.

Goudarzi MH, Eagles DA, Lim J, Biggs KA, Kotze AC, Ruffell AP, Fairlie DP, King GF, Walker AA, Venom composition and bioactive RF-amide peptide toxins of the saddleback caterpillar, Acharia stimulea (Lepidoptera: Limacodidae). Biochem Pharmacol, 213():115598(2023) [pubmed]

submitter keyword: caterpillar, venom, Acharia stimulea

Glenn F. King
contact affiliation	Institute for Molecular Bioscience, The University of Queensland, Australia
contact email	glenn.king@imb.uq.edu.au
lab head
Mohaddeseh Goudarzi
contact affiliation	The University of Queensland
contact email	ihedy.m@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD039980

PRIDE project URI

• PRIDE
  1. PXD039980
     1. Label: PRIDE project
     2. Name: Saddleback caterpillar venom proteomics