PXD039976

PXD039976 is an original dataset announced via ProteomeXchange.

Title	Low-dose Cu exposure enhanced α-synuclein accumolation associates with mitochondtial impairments in mouse model of Parkinson's disease
Description	Parkinson's disease (PD) is a neurodegenerative disorder that mainly affects the elder population, and its etiology is enigmatic. Both environmental risks and genetics may influence the development of PD. Excess copper causes neurotoxicity and accelerates the progression of neurodegenerative diseases. However, the underlying mechanisms of copper-induced neurotoxicity remain controversial. In this study, A53T transgenic α-synuclein (A53T) mice and their matching wild-type (WT) mice were treated with a low dose of copper (0.13 PPM copper chlorinated drinking water) for 4 months, and copper poisoning was performed on human A53T mutant SHSY5Y cells overexpressed with α-synuclein (dose of 1/4 IC50), to test the effects of copper exposure on the body. The results of the open field test showed that the moto function of Cu-treated mice was impaired. Proteomics revealed changes in neurodevelopment, transport function, and mitochondrial membrane-related function in Cu-treated WT mice, which were associated with reduced expression of mitochondrial complex (NDUFA10, NDUFS1, COX5A, ATP5A), dopamine neurons (TH), and dopamine transporter (DAT). Mitochondrial function, nervous system development, synaptic function, and immune response were altered in Cu-treated A53T mice. These changes were associated with increased mitochondrial splitting protein (Drp1), decreased mitochondrial fusion protein (OPA1, Mfn1), abnormalities in mitochondrial autophagy protein (LC3BII/I, P62), decreased dopamine neuron (TH) expression, increased α-synuclein expression, inflammatory factors (IL-6, IL-1β, and TNF-α) release and microglia (Iba1) activation.
HostingRepository	iProX
AnnounceDate	2023-02-09
AnnouncementXML	Submission_2023-02-08_21:55:37.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jie Chen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-02-08 21:55:29	ID requested
⏵ 1	2023-02-08 21:55:37	announced

Dataset with its publication pending

submitter keyword: Parkinson's disease, Copper, Neurodegenerative, Mitochondrial, α-synuclein

Xifei Yang
contact affiliation	Shenzhen Center for Disease Control and Prevention
contact email	xifeiyang@gmail.com
lab head
Jie Chen
contact affiliation	School of Public Health, Xinjiang Medical University&Shenzhen Center for Disease Control and Prevention
contact email	841549379@qq.com
dataset submitter

iProX dataset URI